SKU: 44440804414

ZymoScript One-Step RT-qPCR Kit

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Description

ZymoScript One-Step RT-qPCR KitZymoScript One Step RT qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room temperature reaction setup is quick and easy. The ZymoScript One Step RT qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination. The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure

ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.

The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).

The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RNA quantification. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).

Zymo Research is ready to support your RNA studies and help simplify your RNA quantification workflow. Please contact [email protected] for more information.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
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  • To process your return/exchange, please contact us at [email protected]
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SKU: 44440804414

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4.9 ★★★★★
Based on 22 reviews
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Helena
Louisville, US
★★★★★ 5
These are definitely indestructible
Size: Medium
Both of my dogs can destroy a ball in a matter of minutes if I let them play with them (supervised) as is. Well not with these balls. They are the ONLY ones I have found that are 100% indestructible. Please only let your dogs play with these as a toy when supervised though. They go really far when throwing them, so both dogs get a good amount of running and exercise with these. I also bought these because they have the hole in the middle of the ball which is very important in the event that a dog gets one stuck at the back of their throat. This allows them to breath unlike those without this hold. It's an unlikely event, but as dog owners, we all need to be aware of this and educate ourselves on what to do if this SHOULD happen. This isn't a scare tactic, nor a reflection of this particular brand, it can happen with any type of ball. My two dogs are about 45ish lbs and the size is perfect for them, I don't want anything smaller than this. They love them. There is no annoying squeak. Really easy to wash after use. Some balls take forever to get the slimy saliva off, but these don't.
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Reviewed in the United States on September 25, 2025
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Verified Purchase
Ale quintero
Pawtucket, US
★★★★★ 5
He loves this ball
Size: Medium, Size: Medium
My dog loves this ball. It’s the perfect size, he enjoys playing fetch and this ball has been his fav one. Thank you guys for making my lil one happy
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Reviewed in the United States on May 2, 2026
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Verified Purchase
Alicia
Fort Morgan, US
★★★★★ 5
Bulky
I was a little disappointed in how bulky these were and seemed hard to get on. If you can get past this they do seem to work good. Hands do seem softer and a bit healthier.
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Reviewed in the United States on November 8, 2024
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Verified Purchase
Deb
Natrona Heights, US
★★★★★ 5
Really moisturizes hands
These gloves really work. My cuticles and hands alway seem dry. I used these gloves the first night I received them and my hands were really smooth afterward. When I get in bed at night, I play on my computer and iPad, and these gloves are perfect. The end of the index finger is designed to be used with a phone, computer, and iPad. Make sure you push the end of the fingers on the gloves all the way down to the end of your fingertips; otherwise you will not be able to use them with a device. I find they work better with my iPad than my computer. I have not tried to use with my phone. The gloves are not uncomfortable, but I found they are not comfortable to wear all night, so I just try to wear them about an hour every night. I wish I had these gloves about 20 years ago and my hands would probably look like a 20 year old.
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Reviewed in the United States on May 25, 2024
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phoenix329
Boise, US
★★★★★ 1
So much pain
I had to remove these after less than 5 minutes as they caused such intense pain. The gel clings to your skin even with the lotion and tightens around your hands cutting off circulation. They have been off for about half an hour and my hands still hurt. I was so excited as I really like this brand in general. Very disappointed. Be very aware when buying.
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Reviewed in the United States on October 12, 2023

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