SKU: 44440804414

ZymoScript One-Step RT-qPCR Kit

Sale price$90.86 Regular price$100.95
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Ships within 48 hours · Estimated delivery Sep 27 - Oct 2

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Description

ZymoScript One-Step RT-qPCR KitZymoScript One Step RT qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room temperature reaction setup is quick and easy. The ZymoScript One Step RT qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination. The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure

ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast and robust RNA quantification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.

The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).

The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RNA quantification. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).

Zymo Research is ready to support your RNA studies and help simplify your RNA quantification workflow. Please contact [email protected] for more information.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
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  • To process your return/exchange, please contact us at [email protected]
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SKU: 44440804414

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Janice A
Massapequa, US
★★★★★ 5
My puppy’s favorite
Color: Orange
Great quality and built to last. My dog really enjoys playing with it and stays entertained for quite a while. The material is strong yet safe, and the included drawstring bag is a convenient extra for easy storage. Is colorful and my become my puppy’s favorite.
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Reviewed in the United States on March 1, 2026
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Amazon Customer
Draper, US
★★★★★ 1
If you love your dog…don’t buy this.
Color: Orange
We have a nonaggressive English cocker. She opened the ball every time she played with it. And yes, we are closing it as hard as we can. I do not recommend this toy for any dog! This is a dangerous toy for a dog that is not supervised.
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Reviewed in the United States on March 16, 2026
J
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jeff c
West Palm Beach, US
★★★★★ 4
Keeps your dog busy
Color: Blue
Good toy to keep your dog busy. Make sure you check it periodically to ensure it stays together.
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Reviewed in the United States on April 3, 2026
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OLINDA QUEZADA 🌸
New York, US
★★★★★ 5
dog ball
Color: Blue
This ball is perfect for keeping my dog ​​active and entertained. It's made of strong, durable material, ideal for games of fetch. It doesn't break easily and is light enough for him to carry in his mouth without any problem.
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Reviewed in the United States on February 23, 2026
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Verified Purchase
john wenner
Birmingham, US
★★★★★ 2
Fun but not for rough play.
Color: Orange
The ball contented our “golden” Jaxson for about 15 minutes. He then thought it was a regular ball and it’s just a little delicate for a big dog. I just wrote it off as a loss. Great idea but should be more durable.
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Reviewed in the United States on April 7, 2026

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