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Ships within 48 hours · Estimated delivery Jul 27 - Aug 1
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Description
OneStep PLUS qMethyl PCR KitThe OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite free procedure for the quantification of DNA methylation at custom selected genomic regions. This bisulfite free assessment is accomplished by splitting the DNA to be tested into two parts: a Test Reaction and a Reference Reaction. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The
The OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite-free procedure for the quantification of DNA methylation at custom-selected genomic regions. This bisulfite-free assessment is accomplished by splitting the DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both reactions is then amplified using Real-Time PCR. The difference in Cycle Threshold (Ct) values between the Reference and Test reactions is used to calculate the percentage of DNA methylation at the selected genomic region.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy