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Description
YPD PlusThe outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which normally results in increased cell survival. This is useful when attempting to maximize transformation efficiencies for library screening or transforming yeast
The outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which normally results in increased cell survival. This is useful when attempting to maximize transformation efficiencies for library screening or transforming yeast with multiple plasmids. YPD Plus is a special formulation of conventional YPD outgrowth medium that increases yeast transformation efficiencies by > 50%. YPD Plus is recommended for mutant yeast strains exhibiting poor growth characteristics that are not amenable to transformation. Simply supplement a yeast transformation reaction mixture with YPD Plus to achieve consistent increases in yeast transformation efficiencies.Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
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- Standard Shipping : 3-10 business days
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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4.3 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 1
Very cheaply made !!!!!
Not the color as shown and very small. Could not make it squeak!! Not worth the price I was charged !!!
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Reviewed in the United States on July 30, 2025
★★★★★ 3
Okay, but not indestructible!!
Sharky was a favorite but, not in one piece. Dog loved the toy but it didn't last all that long and we are still finding pieces under the couch/bed/etc. -kelly
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Reviewed in the United States on March 27, 2025
★★★★★ 5
Monster Mutt Loves it
One of the Guardians of our home ranch adores her. First thing she grabs after waking in the morning. Lila Mae enjoys the shape as well as texture. Considering it keeps the South African queen happy and content. Compared to the Boers past toys which they easily bored of easily, the fem stays dillegent in her quest to capture Jaws. Thanks for reading. Postscript, my jacks have no interest. They can't destroy it
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Reviewed in the United States on July 2, 2024
★★★★★ 2
For aggressive chewers.
The product lasted all of 20-40 min. I wasn't timing him chewing. But he was quick to get off the head. He had fun but he must be in a different category than aggressive chewer.
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Reviewed in the United States on July 12, 2024
★★★★★ 5
Great toy!
Color: Blue
This is great and the dog loves it!
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Reviewed in the United States on April 30, 2026