Zymo-Seq SPLAT DNA Library Kit

Zymo-Seq SPLAT DNA Library KitThe Zymo Seq SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell free DNA, and even FFPE derived DNA with ease. Additionally, the SPLAT technology allows for direct ligation of adapters onto the native ends of each DNA fragment,

The Zymo-Seq™ SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single-stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell-free DNA, and even FFPE-derived DNA with ease. Additionally, the SPLAT technology allows for direct ligation of adapters onto the native ends of each DNA fragment, simultaneously eliminating the need for end-repair and ensuring preservation of all original nucleotides. This feature allows for accurate and high-fidelity analysis of fragmentation patterns, setting a new standard for fragmentomics analysis.

The Zymo-Seq™ SPLAT DNA Library Kit easily generates library from 10 ng to 500 ng of pre-fragmented DNA input in just two simple steps: (a) ligation of the single-stranded DNA with the unique splinted adapters concurrently and (b) amplification of the libraries via PCR using unique dual indexes. By utilizing a straightforward workflow that is efficient and user-friendly, the Zymo-Seq™ SPLAT DNA Library Kit allows for generation of high-quality libraries ready for DNA sequencing in as little as 3 hours.