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Description
Rat XBP1 (X-Box Binding Protein 1) ELISA KitThe Rat (XBP1) X Box Binding Protein 1 ELISA Kit measures X Box Binding Protein 1 in Rat samples. The plate has been pre coated with Rat XBP1 antibody. XBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat XBP1 Antibody is added and binds to XBP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated XBP1 antibody. After incubation unbound Streptavidin HRP is washed away during
The Rat (XBP1) X-Box Binding Protein 1 ELISA Kit measures X-Box Binding Protein 1 in Rat samples. The plate has been pre-coated with Rat XBP1 antibody. XBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat XBP1 Antibody is added and binds to XBP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated XBP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat XBP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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