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Description
Mouse SEMA3D (Semaphorin-3D) ELISA KitThe Mouse (SEMA3D) Semaphorin 3D ELISA Kit measures Semaphorin 3D in Mouse samples. The plate has been pre coated with Mouse SEMA3D antibody. SEMA3D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA3D Antibody is added and binds to SEMA3D in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA3D antibody. After incubation unbound Streptavidin HRP is washed away during
The Mouse (SEMA3D) Semaphorin-3D ELISA Kit measures Semaphorin-3D in Mouse samples. The plate has been pre-coated with Mouse SEMA3D antibody. SEMA3D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA3D Antibody is added and binds to SEMA3D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA3D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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