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Description
Human ATG12 (Ubiquitin-like Protein Atg12) ELISA KitThe Human (ATG12) Ubiquitin like Protein Atg12 ELISA Kit measures Ubiquitin like protein ATG12 in samples. The plate has been pre coated with Human ATG12 antibody. ATG12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATG12 Antibody is added and binds to ATG12 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ATG12 antibody. After incubation unbound Streptavidin HRP is
The Human (ATG12) Ubiquitin-like Protein Atg12 ELISA Kit measures Ubiquitin-like protein ATG12 in samples. The plate has been pre-coated with Human ATG12 antibody. ATG12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATG12 Antibody is added and binds to ATG12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATG12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATG12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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