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Description
Rat HBB (Hemoglobin Subunit Beta-1) ELISA KitThe Rat (HBB) Hemoglobin Subunit Beta 1 ELISA Kit measures Hemoglobin subunit beta 1 in Rat samples. The plate has been pre coated with Rat HBB antibody. HBB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HBB Antibody is added and binds to HBB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HBB antibody. After incubation unbound Streptavidin HRP is washed away during a
The Rat (HBB) Hemoglobin Subunit Beta-1 ELISA Kit measures Hemoglobin subunit beta-1 in Rat samples. The plate has been pre-coated with Rat HBB antibody. HBB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HBB Antibody is added and binds to HBB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HBB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HBB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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