SKU: 20756615900

Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit

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Ships within 48 hours · Estimated delivery Sep 11 - Sep 16

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Description

Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA KitThe Human HSP 27 HSPB1 (Heat Shock Protein 27) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human HSP 27 HSPB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP 27 HSPB1 and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-27/HSPB1. You can calculate the concentration of Human HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.

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SKU: 20756615900

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4.3 ★★★★★
Based on 28 reviews
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SunshineSummerSunset
Natrona Heights, US
★★★★★ 5
The Purple Power Chew My Dog Thinks Is His Life’s Mission
I bought this purple rubber bone hoping it would survive longer than the usual 10 minutes my dog needs to destroy a “durable” toy. Shockingly, this one is still alive and thriving. Honestly, I think it might outlive me. The material is tough enough for aggressive chewers, but still bouncy and fun, which means my dog is entertained AND my furniture gets a break. It’s the perfect size for medium to large dogs, and it keeps mine busy like he’s working an important full-time job. He tosses it, chases it, gnaws on it with total devotion… and somehow it barely shows a scratch. The purple color is a bonus, easy to spot in the yard, and it makes him look very fashionable in his ferocious-chewer era. If your dog believes every toy is a challenge to conquer, this chew toy just might be the worthy opponent they’ve been waiting for. 💜🐶
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Reviewed in the United States on December 5, 2025
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Verified Purchase
Ma Ma
Alexandria, US
★★★★★ 3
Not my husky's favorite
Size: Large
No bounce, it's too hard and heavy. My husky only uses it once in a while, it is not his favorite thing to chew on.
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Reviewed in the United States on February 28, 2026
B
Betty
Phoenix, US
★★★★★ 4
Better Than Most, But Still No Match for My Super Chewer
This is a decent chew. It’s made of a softer rubber material and has little to no odor, which I appreciate. It’s been about a week, and while it’s still technically in one piece, it’s starting to show some cracks and tiny puncture holes. Now, keep in mind, my 100-lb pit mix Scooby Doo is a full on chew and destroy hound. If it can be chewed, he’ll find a way to shred it to bits. This toy held up longer than a lot of others but still didn’t make the “indestructible” cut in our house. He likes it, but it’s not his top pick, probably why it’s still alive. Overall, it’s a good toy for the price. Probably not for extreme power chewers like Scoob, but it’ll hold up fine for most dogs and give you a little peace and quiet for a while. Do give it a try, Scooby Doo is a chewer in a class of his own, the fact that this has held up past a one 15 minute session is amazing in itself.
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Reviewed in the United States on November 5, 2025
R
Ray
Chelsea, US
★★★★★ 5
Absolutely thrilled with this
Great price Great find It is heavy but my pup is easily able to carry it around. Perfect for smearing treats on and freezing - keeps him busy for ages - especially with the ridges - thrilled with this
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Reviewed in the United States on December 5, 2025
G
GigiThinks
Charlottesville, US
★★★★★ 5
Multifunctional dog toy, fetch, chew, dental health
This toy feels like soft rubber, unlike the super hard plastic Size wise, it would be a good toy to toss and play fetch with. My Aussiedoodle took to it right away. It has numerous nubs that are great for dental health. I use it, as well as other toys with nubs, to offer to my pups with a bit of smeared on it, to help with their dental health. 🤗 Thank you so much for taking the time to read this review, have a FANTASTIC day!!😊 5★
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Reviewed in the United States on December 27, 2025

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