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Description
Rat SIRT3 (NAD-dependent protein deacetylase sirtuin-3) ELISA KitThe Rat SIRT3 (NAD dependent protein deacetylase sirtuin 3) ELISA Kit measures SIRT3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti SIRT3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP
The Rat SIRT3 (NAD-dependent protein deacetylase sirtuin-3) ELISA Kit measures SIRT3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti SIRT3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with SIRT3 bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SIRT3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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