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Description
Mouse VIM (Vimentin) ELISA KitThe Mouse (VIM) Vimentin ELISA Kit measures Vimentin in Mouse samples. The plate has been pre coated with Mouse VIM antibody. VIM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse VIM Antibody is added and binds to VIM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated VIM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
The Mouse (VIM) Vimentin ELISA Kit measures Vimentin in Mouse samples. The plate has been pre-coated with Mouse VIM antibody. VIM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse VIM Antibody is added and binds to VIM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse VIM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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