Bovine LL-37 (Antibacterial Protein LL-37) ELISA Kit

Bovine LL-37 (Antibacterial Protein LL-37) ELISA KitThe Bovine LL 37 (Antibacterial Protein LL 37) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Bovine LL 37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine LL 37 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to

The Bovine LL-37 (Antibacterial Protein LL-37) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Bovine LL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine LL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Bovine LL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Bovine LL-37. You can calculate the concentration of Bovine LL-37 in the samples by comparing the OD of the samples to the standard curve.