Mouse IL-18 (Interleukin 18) ELISA Kit

Mouse IL-18 (Interleukin 18) ELISA KitThis Mouse IL 18 (Interleukin 18) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse IL 18. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL 18 are added to the micro ELISA plate wells. Mouse IL 18 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL 18.

This Mouse IL-18 (Interleukin 18) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-18 are added to the micro ELISA plate wells. Mouse IL-18 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-18.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IL-18. The concentration of Mouse IL-18 in the samples is then determined by comparing the OD of the samples to the standard curve.