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Description
Rat S100A9 (Protein S100-A9) ELISA KitThe Rat (S100A9) Protein S100 A9 ELISA Kit measures Protein S100 A9 in Rat samples. The plate has been pre coated with Rat S100A9 antibody. S100A9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A9 Antibody is added and binds to S100A9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated S100A9 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Rat (S100A9) Protein S100-A9 ELISA Kit measures Protein S100-A9 in Rat samples. The plate has been pre-coated with Rat S100A9 antibody. S100A9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A9 Antibody is added and binds to S100A9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated S100A9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat S100A9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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