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Ships within 48 hours · Estimated delivery Jul 13 - Jul 18
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Description
Human SEMA3F (Semaphorin 3F) ELISA KitThe Human (SEMA3F) Semaphorin 3F ELISA Kit measures Semaphorin 3F in samples. The plate has been pre coated with Human SEMA3F antibody. SEMA3F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA3F Antibody is added and binds to SEMA3F in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA3F antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (SEMA3F) Semaphorin 3F ELISA Kit measures Semaphorin 3F in samples. The plate has been pre-coated with Human SEMA3F antibody. SEMA3F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA3F Antibody is added and binds to SEMA3F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA3F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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