SKU: 46770943120

Mouse S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit (Small Sample)

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Description

Mouse S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit (Small Sample)The Mouse S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse S1PR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse S1PR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB

The Mouse S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S1PR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S1PR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S1PR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 46770943120

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4.2 ★★★★★
Based on 13 reviews
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K
Verified Purchase
Katie
Lexington, US
★★★★★ 5
Great Counting Book!
Format: Hardcover
This is a really neat counting book. It's all just beautiful pictures. There's so many things to look at and count and it also helps will telling the time at the hour level on an analog clock. I thought it would be hard to hold my kindergartner's interest with it, as he gets tired of all the number and alphabet books. He loved this one. I think not having words let him feel in control of explaining what each two-page spread was about, each a different number.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 26, 2025
M
Verified Purchase
Michele
Omaha, US
★★★★★ 5
Great for interacting with child about numbers
Format: Hardcover
No words. Just beautifully illustrated which encourages child and parent to talk about the story and numbers. It is best for 2 to 4 year olds.
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Reviewed in the United States on November 10, 2025
D
Verified Purchase
dhoped123
Draper, US
★★★★★ 5
Beautiful book for children
Format: Hardcover
My children have two of Anno's books, this one as well as Anno's Italy. As an adult, I find the books beautiful in a very clean and simple way. The illustrations in Anno's Counting Book have a very classic look, and there are no words at all. I'm always surprised that my kids continue to request this book at bedtime, since there is really no story. The idea is pretty simple: there are 12 scenes, and scene number one has one of each item. Scene two has two of each item, etc. It took us a minute to realize that the 12 scenes also represent the 12 months of the year. What makes the book work is really the simplicity and universality of the illustrations. It's great for younger kids in particular. My three year old just recently started understanding what numbers and counting really mean, so this book is a great way to continue developing his number sense. My five year old gets excited to pick items on each page to count. He's convinced he's going to find the wrong number of items on one of the pages (though he hasn't yet). My seven year old has mostly lost interest at this point.
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Reviewed in the United States on March 13, 2017
C
Verified Purchase
Chingyuan
Whiting, US
★★★★★ 5
Highly recommended!
Format: Hardcover
I first saw this book at a relative’s house and was immediately drawn in by its content. There’s no text at all, yet it captures children’s interest so strongly—especially when learning the concept of numbers. The illustrations are also beautiful. When my child didn’t yet understand numbers, it was still fun to recognize the animals or see what the characters in the pictures were doing. I really love this book.
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Reviewed in the United States on September 14, 2025
K
Verified Purchase
KB
Cuba, US
★★★★★ 5
A Multi-faceted, beautiful book for babies through primary grades.
Format: Hardcover
Our second generation is now loving this beautiful, clever, playful book. Although there are no words, the story line is a delight. A watercolored village develops one page to the next starting with 0 and ending with 12. The pictures add one item in each category for each new number, building on the previous scene. Readers can find and count buildings, children, adults, pine trees, cherry trees, and an assortment of animals. In addition, in a lovely subtle manner, the four northern hemisphere seasons pass from January to December. The details of adult and child activities through time and seasons can be noted. Anno's creativity in this genre is unsurpassed. For my children, and now for my grandson, this book evokes fascination on several levels both aesthetic and intellectual. Start using it as a baby word book, and it will last as a joy throughout early childhood. Not every baby book must be a board book. Teaching children to turn pages gently is easy to do and adds to the amazing, shared experience.
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Reviewed in the United States on February 22, 2015

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