QuicKey Pro Mouse CORT (Corticosterone) ELISA Kit

QuicKey Pro Mouse CORT (Corticosterone) ELISA KitWith about 20 known citations, the QuicKey Pro Mouse CORT (Corticosterone) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites

With about 20 known citations, the QuicKey Pro Mouse CORT (Corticosterone) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.