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Description
Human Anti-HER2/ERBB2 (Receptor tyrosine-protein kinase erbB-2) antibody ELISA KitThe Human Anti HER2 ERBB2 (Receptor tyrosine protein kinase erbB 2) antibody ELISA Kit measures HER2 ERBB2 Ab in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP
The Human Anti-HER2/ERBB2 (Receptor tyrosine-protein kinase erbB-2) antibody ELISA Kit measures HER2/ERBB2 Ab in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HER2/ERBB2 Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HER2/ERBB2 Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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