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Description
Rat MT3 (Metallothionein 3) ELISA KitThe Rat MT3 (Metallothionein 3) ELISA Kit measures MT3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti MT3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antibody, then it
The Rat MT3 (Metallothionein 3) ELISA Kit measures MT3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti MT3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with MT3 bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MT3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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