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Description
Human DIO3 (Thyroxine 5-Deiodinase) ELISA KitThe Human (DIO3) Thyroxine 5 Deiodinase ELISA Kit measures Thyroxine 5 Deiodinase in samples. The plate has been pre coated with Human DIO3 antibody. DIO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DIO3 Antibody is added and binds to DIO3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DIO3 antibody. After incubation unbound Streptavidin HRP is washed away during
The Human (DIO3) Thyroxine 5-Deiodinase ELISA Kit measures Thyroxine 5-Deiodinase in samples. The plate has been pre-coated with Human DIO3 antibody. DIO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DIO3 Antibody is added and binds to DIO3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DIO3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DIO3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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