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Description
Mouse PAH (Phenylalanine-4-hydroxylase) ELISA KitThe Mouse (PAH) Phenylalanine 4 hydroxylase ELISA Kit measures Phenylalanine 4 hydroxylase in Mouse samples. The plate has been pre coated with Mouse PAH antibody. PAH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PAH Antibody is added and binds to PAH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PAH antibody. After incubation unbound Streptavidin HRP is washed
The Mouse (PAH) Phenylalanine-4-hydroxylase ELISA Kit measures Phenylalanine-4-hydroxylase in Mouse samples. The plate has been pre-coated with Mouse PAH antibody. PAH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PAH Antibody is added and binds to PAH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PAH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PAH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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