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Description
Kidney Injury Molecule 1 (KIM-1) ELISAThe Kidney Injury Molecule 1 (KIM 1) ELISA is an enzyme immunoassay for the quantitative determination of Kidney Injury Molecule 1 (KIM 1) in human urine and serum. The microtiter plate is coated with the antibody specifically binding the Kidney Injury Molecule 1. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP labelled detection
The Kidney Injury Molecule 1 (KIM-1) ELISA is an enzyme immunoassay for the quantitative determination of Kidney Injury Molecule 1 (KIM-1) in human urine and serum.
The microtiter plate is coated with the antibody specifically binding the Kidney Injury Molecule 1. The human serum or plasma is incubated in the plate with the capture antibody.The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of KIM-1 in the specimen. The concentration of KIM-1 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
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