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Description
Mouse CNTF (Ciliary neurotrophic factor) ELISA KitThe Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit measures Cntf in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Cntf antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
The Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit measures Cntf in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Cntf antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Cntf bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Cntf in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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