Rat MHCII/AgBII/H-1II (Major Histocompatibility ComplexII) ELISA Kit

Rat MHCII/AgBII/H-1II (Major Histocompatibility ComplexII) ELISA KitThe Rat MHCII AgBII H 1II (Major Histocompatibility ComplexII) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat MHCII AgBII H 1II. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCII AgBII H 1II and Avidin Horseradish Peroxidase (HRP)

The Rat MHCII/AgBII/H-1II (Major Histocompatibility ComplexII) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MHCII/AgBII/H-1II. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCII/AgBII/H-1II and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MHCII/AgBII/H-1II, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MHCII/AgBII/H-1II. You can calculate the concentration of Rat MHCII/AgBII/H-1II in the samples by comparing the OD of the samples to the standard curve.