SKU: 97812823635

Porcine OFQ/N (Orphanin FQ/Nociceptin) ELISA Kit

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Description

Porcine OFQ/N (Orphanin FQ/Nociceptin) ELISA KitThe Porcine OFQ N (Orphanin FQ Nociceptin) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Porcine OFQ N. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Porcine OFQ N. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate

The Porcine OFQ/N (Orphanin FQ/Nociceptin) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine OFQ/N. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Porcine OFQ/N. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Porcine OFQ/N in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 97812823635

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