Human Ang-II (Angiotensin II) ELISA Kit

Human Ang-II (Angiotensin II) ELISA KitWith about 13 known citations, the Human Ang II (Angiotensin II) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human Ang . During the reaction, Human Ang in the sample or standard competes with a fixed amount of Human Ang on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang . Excess conjugate and unbound sample or standard are washed away,

With about 13 known citations, the Human Ang-II (Angiotensin II) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-â…¡. During the reaction, Human Ang-â…¡ in the sample or standard competes with a fixed amount of Human Ang-â…¡ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-â…¡. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-â…¡ in tested samples can be calculated by comparing the OD of the samples to the standard curve.