Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 29 - Sep 3
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse IRG1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an immunoreactive gene 1 (IRG1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of immunoreactive gene 1 (IRG1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Immunoresponsive gene 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Immune response gene protein 1 (IRG1) is a metabolic enzyme in the tricarboxylic acid cycle that catalyzes the decarboxylation of aconitic acid to itaconate. It encodes a protein involved in pathways including glucose and energy metabolism. IRG1/itaconate is a crucial link between macrophage immunity and metabolism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 12 reviews
Sort
Product Reviews
★★★★★ 5
Own This Book Along With A Visit To The Museum
Format: Hardcover
This book tells the story of the founding and exhibits of the Holocaust Museum in Washington, DC. This book does not replace visits to the Museum. It is an excellent reference book to have in combination with visits to the Museum. The Holocaust Museum is one of the finest Museums in Washington, DC.
The history told at the Museum is powerful in so many ways. My hope is that humanity can learn from history not to keep repeating the many horrific mistakes that were made during the period of Hitler and Stalin. We must always remember that most of the terrible crimes during this period were sanctioned, promoted and authorized by National Governments. The Governments of nations can and do become evil, corrupt and murderous. We the people of the world must be forever vigilant against vesting too much power in the hands of too few people. Concentrated power almost always becomes corrupt.
We must learn from history how to prevent the concentrations of power in too few people. These concentrations of power can sooner or later lead to evil. Even power that is initially built on good intentions will good bad eventually. That is the nature of the human condition that we must fear that charismatic leaders will concentrate power in ways that are unhealthy and deadly. We should find ways to avoid electing charismatic leaders as these types of leaders are just too dangerous. The Holocaust happened just seconds ago in terms of the lifespan of our planet, and something like it will happen again unless we learn the lessons taught to us by history.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2005
★★★★★ 4
Book was not Hardcover
Format: Hardcover
In the description it says it is hardcover but when book arrived it was a paperback book. Book was suppose to be new but spine of book has stretched out and a small tear on back cover. I have sent book back. Really want hardcover because it is a gift for my mother.
It looked like it was a beautiful book and if it does not matter to you if it is Paperback by all means order this book.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 7, 2019
★★★★★ 5
Five Stars
Format: Hardcover
wonderful book
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 6, 2014
★★★★★ 5
This book will make a difference in your presentation.
Format: Paperback
If you rely on Powerpoint-like presentation in your work, get Cliff Atkinson's Beyond Bullet Points. I had determined that improving my company's presentations had potentially very high returns so I started poking around the net and Amazon for resources to help. At first, I struck out with books that were supposed to improve presentations, but ended up being guides on technically how to use Powerpoint. That was NOT what I was looking for.
Beyond Bullet Points is very different. It is a philosophy about creating presentations whose purpose is to communicate a story, not dump information. Frankly, it was not intuitive for me so I had to decide to just trust that it would work. When I was about ¾ finished, I started to really "feel" what I was doing. And, to my surprise, the most unlikely people really liked the result.
In a world where most business and how-to books are nothing more that restating what you already know (or, what you know isn't true), this one is an exception. I highly recommend it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 19, 2005
★★★★★ 5
Presentations with the audience in mind
Format: Paperback
I bought this hoping it would be a guide to creating Big-3 Consulting-style slides with fancy diagrams and eye-catching graphic design. That is not what this book is. Instead, this book is about how to tell a story with slides, using the framework of a five act drama. With this method forcing you to focus on who your audience is, what they need to know, and how much time you have to tell them (as opposed to trying to shoehorn everything you know about a subject into your pitch) you end up with a presentation that finishes on time for intelligent questions from an engaged audience.
Since buying the book I have given several well-received presentations using precisely that technique. With no words on screen there is no temptation to just read the bullet points, and the audience cannot think ahead of you and must instead listen to what you're saying. You have to know your material to use this method, but when you succeed your audience will be impressed with your knowledge of the subject matter. (If you're working in a group project and want to Blue Falcon a non-contributing teammate, try giving them a few of these slides to speak to.)
The recommended slide format is one picture and one headline per slide, with no bullet points at all. The book suggests creating Notes Pages with an outline of your talk as a handout, since the slides themselves don't stand alone. (And that's a good thing--visual aids are supposed to *augment* the presentation, not *be* the presentation.)
In conclusion, this book might not be for everyone, but it was exactly what I needed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 15, 2018
recommand products
Tama HP9007R Regular Pedal Spring
10.99
Ibanez IAB541BE PowerPad Designer Acoustic Guitar Gig Bag - Beige
104.99
44, Lille, City With Number, Women's Short Sleeve Round White T-shirt 00008
20.00
44, Bradford, City With Number, Women's Short Sleeve Round White T-shirt 00008
20.00
Custom White Navy Hockey Jersey
29.99