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Description
Human CCDC39 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against coiled-coil domain-containing protein 39 (CCDC39). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of coiled-coil domain-containing protein 39 (CCDC39) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Coiled-coil domain-containing protein 39 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Coiled-Coil Domain Containing 39 is a protein-coding gene. Diseases associated with CCDC39 include ciliary dyskinesia, primary, 14, and primary ciliary dyskinesia. Gene Ontology (GO) annotations associated with this gene include nucleotide binding. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.3 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Great for Sensitive Skin
Style: SPF 30, Size: 5 Fl Oz (Pack of 1)
I've been using greasy white, mineral sunscreens for years because the chemical sunscreens all made me break out in a rash--until I tried this one. It dries clear, is non-greasy, and goes on well. Note the relatively few inactive ingredients compared to other chemical sunscreens. Whatever ingredient irritated my skin is not in this one and I love it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 30, 2023
★★★★★ 1
Expired
Style: SPF 30, Size: 5 Fl Oz (Pack of 1), Style: SPF 30, Size: 5 Fl Oz (Pack of 1)
I just received this item which was ordered specifically 17th of December and I received it on December 20th. I was excited and didn't bother to read the reviews and wish I had. To begin the expiration is December 2024, therefore this product I just received is basically useless. Secondly to my eye the product came battered, there seem to be some marks on the bottle and if I have to be honest to me looks as if it has been used. Given the dangers potential dangers of using expired skin product I will not be using this, and the worst thing of all, this item is not returnable, going to figure out if I can get a refund at least.
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Reviewed in the United States on December 23, 2024
★★★★★ 3
Expires in only 3 months so that's on seller "The MMC." Otherwise the sunscreen itself is great.
Style: SPF 30, Size: 5 Fl Oz (Pack of 1)
I split the difference on the rating: I would give only 1 star for seller "The MMC" because as others have experienced there is little time left before the product I received expires (3 months). I expect that to really matter on a product where you are relying on the strength of the ingredients to protect your skin. Otherwise I'd give a 5 rating for the sunscreen itself.
I tried 3 different brands of mineral sunscreens in the last few years because I had become concerned about loading up my skin with chemicals. They were all a little "white hazy" but I am fair-skinned so once they were on about 20 minutes they weren't very noticeable. But I had to sit on a towel in my car to protect my upholstery and they all left faint white streaks on the armrests and window sills, and reapplying them later in the day was a pain when I had to worry about getting them on my clothes. The last straw came when Consumer Reports published their research showing very poor scores for all the mineral sunscreens they tested. So I reluctantly switched back to a chemical sunscreen and after some research I settled on this one - Eucerin Sun Advanced Hydration SPF 30. It fared decently in Consumer Reports ratings and the ingredients are rated "low hazard" by the Environmental Working Group. It is definitely a thinner lotion than most sunscreens but I've gotten used to it and now consider it a plus -- it's very quick and easy to apply and it absorbs fast. And it doesn't make a mess on my clothes when I reapply it. Some people have said that it's a little shiny on the skin and that's true. I should note that I use a different sunscreen just for my face and neck and I don't wear makeup anymore so that's not a factor for me. As far as sun protection goes, which is kind of the point, I spent over a week hiking in five national parks in the Utah sun at high altitude this past June and I did not get even a whisper of sunburn despite being fair-skinned. So the product did its job.
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Reviewed in the United States on October 1, 2024
★★★★★ 5
Easy application - absorbs easily
Style: SPF 30, Size: 5 Fl Oz (Pack of 1)
The sunscreen is quite thin and liquidy which made applying it easy and quick.
Spent 2 hours per day for several days playing pickleball in full, constant sun and not a tinge of pink on my skin.
I highly recommend this product!
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Reviewed in the United States on February 25, 2024
★★★★★ 5
Soothing Lotion - Excellent before bedtime
Size: 12 Fl Oz (Pack of 1)
I absolutely love this lotion. The lavender scent is gentle and calming without being overpowering, and it really does make applying it feel like a small moment of relaxation. The texture is smooth and absorbs nicely, leaving my skin soft and well moisturized without feeling greasy. The natural oatmeal formula feels very soothing, especially when skin is dry or irritated. It’s become a staple in my routine because it hydrates so well and the scent is so comforting. A wonderful lotion to keep on hand if you enjoy skincare that both nourishes your skin and helps you unwind a little at the end of the day.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 5, 2026
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