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Description
Human VEGF ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human VEGF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human VEGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference corrected wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGF, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor, is an effective regulator of angiogenesis and angiogenesis in fetus and adults. Vascular endothelial growth factor belongs to the PDGF family, a family of proteins characterized by the presence of eight conserved cystine residues at the structure of the cystine knot and the dimer bound by antiparallel disulfide bonds. After alternative cleavage and sequence length of amino acids, humans express different isoforms, including: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189 and VEGF206, among others. Among them, VEGF165 is the highest expression isoform, followed by VEGF121 and VEGF189. With the exception of VEGF121, the other isoforms contain a basic heparin-binding region and do not diffuse freely. Human VEGF165 has 88% homology to the corresponding protein amino acid sequences of mouse and rat. VEGF is expressed in various cells and tissues, including skeletal muscle cells and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34 + stem cells, endothelial cells, fibroblasts, vascular smooth muscle cells, and the like. The expression of VEGF is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatin M and tumor necrosis factor alpha. The expression level of VEGF is also different during development and in adults. The dimer of VEGF binds to two related tyrosine kinase receptors, namely VEGFR1 (also called Flt-1) and VEGFR2 (Flk-1/KDR). VEGF can induce both homodimerization and autophosphorylation of the latter. These receptors have seven extracellular immunoglobulin domains and one intracellular separate tyrosine receptor domain. Both vascular endothelial cells and some other non-endothelial cells express VEGF receptors. Although VEGF has the highest affinity with VEGFR1, VEGFR2 is the main factor regulating angiogenesis of VEGF. VEGF165 also binds to the semaporin receptor, Neuropilin-1, thereby promoting complex formation with VEGFR2. VEGF is known for its involvement in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, and thus regulates the density and volume of blood vessels. But it doesn't work on the pattern of vascularization. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemokine. Postpartum, VEGF maintains the integrity of vascular endothelial cells and is an effective mitogen for large/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and the female reproductive cycle. In diseased tissues, VEGF promotes vascular permeability. Therefore, VEGF is involved in the metastatic process of tumors through extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control tumor angiogenesis induced by VEGF. The level of VEGF circulating in the body is related to the extent of autoimmune diseases (such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, etc.). |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.7 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 5
Exactly as described and what I needed.
Size: 2' x 6' (Natural), Color: Wolf Tipped
I have a bad back along with other medical problems. I bought this for padding in my wheelchair. Very thick, soft, flexible, everything I needed. No smell and very little shedding even initially. Arrived quickly and well packaged.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 24, 2026
★★★★★ 5
High quality and GORgeous!
Size: 2' x 3' (Sheepskin shape), Color: Creamy Gray, Size: 2' x 3' (Sheepskin shape), Color: Creamy Gray
I was hesitant to order this online but oh my GAWD, it's so much better than I thought it'd be. Soft and warm, and cozy. Far exceeded my expectations, and really took the Eames to the next level. Highly recommend.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Good buy
Size: 2' x 6' (Sheepskin shape), Color: Military Green
Soft , good quality. Rich color. Nice size. No shedding. Does move around.
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Reviewed in the United States on April 24, 2026
★★★★★ 5
Very nice quality
Size: 2' x 3' (Sheepskin shape), Color: Creamy White, Size: 2' x 3' (Sheepskin shape), Color: Creamy White
I ordered three of these, and they arrived promptly and very well packaged in a vacuum seal pack. Once you pull them out, shake them and fluff them a little bit they lose their wrinkles pretty quickly. They seem to be very good quality and are authentically sheepskin. The “white” has a tiny little bit of a yellow undertone, which is what I expected since it’s basically a dyed sheep skin. I would call the white color “natural” rather than white white. They are very thick and were a good price. I ordered two of the three foot-long hides and one 6ft foot hide. One of the hides had a slight odor to it that reminded me a little bit of eggs. It’s been laying on my bench all night and 24 hours later there is no smell. The other two hides had zero smell at all. I think it’s just a little leftover chemical smell from the treatment of the hide when they get it ready. It wasn’t overwhelming or overpowering. If you’re looking for the ultimate SUPER plush sheepskin, I recommend the Overland Sheepskin Company. However, for what I need these for which is just something to throw over my dining room table bench for a little cushion and aesthetics. It’s absolutely perfect and the right price point.
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Reviewed in the United States on February 27, 2025
★★★★★ 5
Beautiful Good Quality Sheepskin, Beautifully Packaged
Size: 2' x 6' (Sheepskin shape), Color: Creamy White, Size: 2' x 6' (Sheepskin shape), Color: Creamy White
Very nicely packaged and presented sheepskin rug. Seems like very good quality and is just as I imagined it would be based on my previous experiences with sheepskin.
I don’t think it’s as soft as others seemed to think but it has been chemically processed and it is very similar in texture to what I remember from past experiences with sheepskin.
I got this for the kitties to nap on - one seemed proud of me for bringing home a pelt and went into hunt mode. The other looked at me suspiciously like he didn’t really know me at all and was afraid I could be eyeing him for his fur!
Hopefully once they both adjust they’ll enjoy napping on it 🙀😻
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Reviewed in the United States on March 19, 2026
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