SKU: 13242670764

Human TNF-α ELISA Kit(Plus)

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Description

Human TNF-α ELISA Kit(Plus)Product Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human TNF &alpha Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake and mix well, and then place it at room temperature for 2 hours of incubation process. The tnf &alpha in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human TNF - &alpha  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake and mix well, and then place it at room temperature for 2 hours of incubation process. The tnf-&alpha in the sample  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and TNF - &alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: pre coated 96 well plates, standards, tnf-α One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 0.42 pg/ml

Detection Range: 15.63-1000pg/ml

Recovery Range: 83-113%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Tumor necrosis factor alpha; (tnf-α)Also known as cachexia and tnfsf1a, it is an adipokine that participates in systemic inflammation and is also one of the cytokines that stimulate the acute phase response. It is mainly produced by activated macrophages, but also secreted by other types of cells, such as cd4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neurons. It has a variety of regulatory functions in the immune response and can also serve as a potential pyrogen. Tnf-α After responding to stimuli (infectious agents or tissue damage), it activates neutrophils in the systemic circulation, changes the characteristics of vascular endothelial cells, regulates the metabolic activity of other tissues, and exhibits tumor killing activity by inducing local coagulation. Tnf-α It plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information shows that tnf-α It is also involved in the pathogenesis of AIDS. Tnf-α The assay is also very useful for transplantation research.

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SKU: 13242670764

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4.6 ★★★★★
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Jerry S. Mefford
Fort Morgan, US
★★★★★ 5
Toss this printer away
Style: WF-3823
Less than a year old and it will not accept Espon print cartridges. I have googled and done everything possible to reboot the printer, but nothing is working
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Reviewed in the United States on January 22, 2026
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Jase T. Wolfe
San Leandro, US
★★★★★ 1
Huge Step Backwards
Style: WF-3823
Purchased this after my WF-3540 finally died. This is a disappointing step backwards in capability for the price. Printing is at best "fine". Getting good color matching / saturation has been a challenge but otherwise acceptable for general things like labels. Despite the confusing documentation (at the time of purchase), this does not do double-sided scanning. This makes using the ADF for multi-sided documents basically pointless. This will be my last Epson. UPDATE - Months have passed and I'm moving it from 2 to 1 star. The ink cartridges run out quickly and are expensive to replace. The ADF jams constantly on scan jobs. The paper pull from the main cassette pulls multiple pages at a time. On top of all that, when using this with a WiFi connection to your network only, the amount of times that it losses connectivity but thinks it is still connected is constant, and/or assigns itself a local IP address instead of getting one from DHCP results in having to cycle power to get it back working. Trash printer.
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Reviewed in the United States on October 23, 2024
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Verified Purchase
Alexsandra Sanchez
Phoenix, US
★★★★★ 4
Happy with my printer!
Style: WF-3823
great buy im super happy with it, i had a HP business printer for about 5 years and I was unhappy with the cost of my $500 ink. So i decided to just buy another printer with a lower cost of ink. I have the same outcome in the printing, great quality, accurate and bright colors. I was able to print business cards and Sticker labels but you can only add 1 sheet to the printer while printing cardstock or labels or it'll jam. But honestly it doesnt matter to me im just happy i dont have to pay so much for ink now. The sheets take a few seconds to print but this is fine for anyone who doesnt need to rush multiple copies. the sheets do shoot out with force when done printing so the sheets do often fall on the floor but if your sitting near you can prevent it. I am able to print wirelessly from both my laptop and iphone!
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Reviewed in the United States on May 8, 2026
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Richard Livingston
Chelsea, US
★★★★★ 5
Prints fast for home use
Style: WF-3823
Great printer and easy to set up, epson should give u the printer for free because they rake u over the coals for ink, Will not work with refurbished cartriges!!!
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Reviewed in the United States on May 2, 2026
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King Arturo
Birmingham, US
★★★★★ 5
Easy Peasy to Set Up
Style: WF-3823
This is probably the sixth printer I've purchased, of which this is the second Epson. My first Epson, which is an earlier WF version, is probably at least ten years old and still operates the same as it did when I first purchased it at least ten years ago. The other three printers were a very well known brand, which I won't mention here, and none survived more than a couple of years each. This brand new Epson WF 3823 took about two weeks to arrive, but it was well worth the wait. It was very easy to set up as it took me all of four minutes to connect to my network and print out my first page. And for $80, I feel like they gave it away.
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Reviewed in the United States on January 12, 2026

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