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Description
UA-Glo® Bright Luciferase Assay SystemProduct Specification Synonyms High luminosity Luciferase Detection Kit Stability & Storage Reagents stored at 20C that cannot be used up completely once opened are recommended to be aliquoted and then frozen at 20C. It is recommended that freeze thaw cycles do not exceed 3 times, and each time the reagent is left at room temperature, the duration should not exceed 1 hour. Background The UA Glo High Luminosity Luciferase Assay Kit is used for the
Product Specification
| Synonyms | High-luminosity Luciferase Detection Kit |
| Stability & Storage |
Reagents stored at 20°C that cannot be used up completely once opened are recommended to be aliquoted and then frozen at 20°C. It is recommended that freeze-thaw cycles do not exceed 3 times, and each time the reagent is left at room temperature, the duration should not exceed 1 hour. |
Background
The UA-Glo High Luminosity Luciferase Assay Kit is used for the quantitative detection of the content of stably expressed luciferase in cells. This reagent features high signal-to-noise ratio, good repeatability, and excellent stability. There is no need to remove the culture medium; the detection reagent can be directly added to the culture plate for detection, thereby further reducing operational errors. Moreover, its stable signal makes this product particularly suitable for high-throughput sample detection. Compared with the Steady Glo stable luciferase assay kit, the Bright Glo high luminosity luciferase assay kit has a detection signal several times higher, which can meet the needs of high-sensitivity detection.
Components
ATP, luciferin, and buffer solution are mixed and then filled into 10 ml or 100 ml brown bottles, with the specifications as follows:
|
Product Specifications |
Can detect the number of wells in a 96-well plate |
Can detect the number of wells in a 384-well plate |
|
10 ml |
100 |
500 |
|
100 ml |
1,000 |
5,000 |
|
10 x 100 ml |
10,000 |
50,000 |
Protocol
1. Before the experiment, equilibrate the UA-Glo high-luminosity luciferase detection reagent to room temperature and mix gently by shaking.
2. Equilibrate the cell plate to be tested (white opaque-bottom or black opaque-bottom plate) at room temperature for 10 minutes.
3. Add the detection reagent in a volume equal to that of the cell culture medium to each culture reaction well.
4. Shake on a plate shaker for 2 minutes, then incubate the sample plate at room temperature in the dark for 5-8 minutes.
5. Read and record the fluorescence signal on a luminescence microplate reader. It is recommended to complete the plate reading within 10-30 minutes to obtain the best results.
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