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Description
Mouse cholinesterase ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are added sequentially to microwells pre-coated with a cholinesterase capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of cholinesterase in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse cholinesterase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cholinesterase, commonly referred to specifically as butyrylcholinesterase (BCHE), is a nonspecific cholinesterase that hydrolyzes many different choline esters. It is produced in the liver and primarily found in plasma, encoded by the BCHE gene. It is very similar to neuronal acetylcholinesterase, also known as RBC or erythrocyte cholinesterase. The term "serum cholinesterase" generally refers to clinical tests that reflect blood levels of both enzymes. Measurement of butyrylcholinesterase activity in plasma serves as a liver function test, as both hypercholinesteremia and hypocholinesteremia indicate pathological processes. BCHE has a half-life of approximately 10 to 14 days. Butyrylcholine is a synthetic compound that does not occur naturally in the body. It is used as a tool to distinguish between acetylcholinesterase and butyrylcholinesterase. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.1 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Canvas crossbody messenger for work, school and travel. Beautiful dark green
This is a great canvas tote messenger crossbody bag. The canvas material ensures that it’s durable and can hold all the things including iPads/laptops and other school/work related items, making this a great school or commuter bag. It’s the perfect size for that type of bag as well as for traveling when you need extra room to carry necessities. I ordered this mainly because I have plenty of fashionable bags, but my husband likes more plain or “gender-free” bags to carry and this one is perfect for boys, men, girls and women and literally anyone needing a satchel to carry their belongings. The crossbody strap is thick and comfortable and creates a super easy wearing experience, where all your things are close and easy to access. I love the dark green color where even though it’s not black/brown it’s still a “neutral” and matches most everything.
Overall it’s a great bag at a great price point that could work for most people needing this style/size. If like me you want to jazz it up a bit, there’s plenty of room to hang bag charmies and chains and the canvas is even easy to hotfix rhinestones to. Would also make a good gift and especially if someone is traveling or moving as I love this size for travel to carry all the extras. I’m very happy with the quality, color, size and comfort.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Spacious, versatile canvas crossbody that hits the sweet spot between purse and messenger bag
I needed something larger than my usual purse but smaller than a messenger bag for days when I need to carry more without going too bulky. This fills that gap perfectly. It could even work as an overnight bag if you travel light.
The main compartment is spacious with two interior compartments layered inside it. One is open pocket style and one has a zipper. There are also two open pockets on the outside. The shoulder strap adjusts easily and should work for most people. The zipper pulls have rectangular holes I can clip keys to, and I added a small clip to the main zipper to secure it to the strap hardware.
The canvas texture means it isn't wipe-clean, but it should wash easily which matters to me. One unexpected and welcome detail: the desiccant pack inside was scented and made the whole bag smell great right from the start, rather than the usual chemical or dye smell new bags often have.
✅ Highlights
• Hits the sweet spot between purse and messenger bag in size
• Interior with multiple compartments
• Zipper pulls have holes for clipping keys or a security clip
• Adjustable strap works for most body types
• Scented desiccant pack means no unpleasant new-bag smell
❌ Cons
• Main zipper requires two hands to close when fully open
• Canvas texture means not wipe-clean
This is a well-thought-out but simple bag that works exactly as described. The minor zipper quirk aside, it does everything I needed it to do.
-Review of GEMIU "GEMIU Canvas Hobo Crossbody Bag for Women Casual Tote Shoulder Bag Large Messenger Bag for Travel"
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Reviewed in the United States on March 15, 2026
★★★★★ 5
A Nice Size Versatile Tote/Bag/Purse
This GEMIU Canvas Hobo Crossbody Bag for Women Casual Tote Shoulder Bag Large Messenger Bag for Travel is a nice versatile tote that can be used as a purse, a work bag, or a travel bag. It is light-weight, made of a medium thick canvas, and can fit a laptop or iPad as well as other items, although it doesn't have the protective cushion for electronics. There are two deep pockets on one outer side, and the other outer side doesn't have pockets, but you can see the stitching from the inner pockets. Inside the bag, there are two pockets on one side; one is zipped and it sits on top of the second open pocket. The main compartment zipper goes beyond the bag so it allows the mouth of the bag to be opened all the way. But with this type of zipper, there is a small opening that remains at the end. The straps are easily adjustable to allow wear as a purse or crossbody. I typically like having smaller purses so I don't have to carry heavy things, but when I go on vacation or visit out of town, it's nice to have a bigger bag to carry things I don't normally carry in my purse (e.g., power bank, umbrella, water bottle, etc.). I can fit my smaller purse into this tote and carry some additional items in it - it's the best of both worlds. The bag does have a slight smell when I first took it out of the plastic package, but it dissipated over time.
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Reviewed in the United States on March 14, 2026
★★★★★ 1
Smells terrible
Color: Army Green
I bought this in green. Love the bag and the color. But the smell!! It is horrible.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Good hobo bag
Color: Grey
I love a good hobo bag for its laid-back style and functionality. It’s spacious without feeling bulky, and the slouchy design gives it that effortless, everyday vibe. There was no odor,material is a good thickness, not sure on durability since I just started to use it. The bag was bigger than I expected.
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Reviewed in the United States on April 22, 2026
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