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Description
Saposin-A His Tag Protein, HumanProduct Specification Species Human Synonyms Proactivator polypeptide; Saposin A; PSAP; GLBA; SAP1 Accession P07602 Amino Acid Sequence Ser60 Ser140, with N terminal His Tag Expression System E. coli Molecular Weight 10kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 20 mM HEPES, 150 mM NaCl, pH 7. 5 Stability & Storage 12 months from date of receipt, lyophilized
Product Specification
| Species | Human |
| Synonyms | Proactivator polypeptide; Saposin-A; PSAP; GLBA; SAP1 |
| Accession | P07602 |
| Amino Acid Sequence | Ser60-Ser140, with N-terminal His Tag |
| Expression System | E.coli |
| Molecular Weight | 10kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM HEPES, 150 mM NaCl, pH 7.5 |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. 2020 Feb;129(2):161-164. Epub 2019 Aug 5. |
Background
Saposin-A is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
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