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Description
Mouse NRG4 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Neuregulin 4 (NRG4) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Neuregulin 4 (NRG4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Neuregulin 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Neuregulins are a family of four structurally related proteins belonging to the EGF protein family. These proteins have been shown to have diverse functions in the development of the nervous system and play a variety of important roles in vertebrate embryonic development, including cardiac development, Schwann cell and oligodendrocyte differentiation, certain aspects of neuronal development, and neuromuscular synapse formation. Neuregulin 4 (NRG4) is a member of the neuroregulin family and is encoded by the NRG4 gene. Neuregulins, including NRG4, activate ERB-B2 receptor tyrosine kinase 4 (ERBB4), initiating cell signaling through membrane tyrosine phosphorylation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Fits Perfect
This fits my cordless P10 Pro perfectly. It has some foam and hard grooves for picking up debris. The part feels very solid, not cheap or low quality at all. Be sure your Dreame is one of the models listed as this only fits those. It won't fit any of the other brands that share some of the Dreame parts (like Mova, for example). The rollers do eventually wear and reduce performance. This is a nice alternative to the name brand.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 19, 2025
★★★★★ 5
Good Replacement Set
Style: 19 Pack
Good replacement set for the price. Parts are basically like the originals and are easily installed for a perfect fit for the 25C.
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Reviewed in the United States on July 11, 2025
★★★★★ 5
Rejuvenates your Robovac, but one caution
I was a little wary buying third-party aftermarket parts for my Eufy Robovac 30c, however, all the parts in this kit are virtually identical to OEM parts. I replaced all three brushes and all three filters and let it loose on my Golden Retriever-inhabited living room. I can honestly say the result was a 50% increase in the amount of fur collected. I suspect that over time there is a loss of performance so gradual that it's easy to not notice. I assume the new filters returned the vacuum power to it's original level of suction was a big factor.
When I was browsing through all the available choices here on Amazon, a plastic "ring" kept appearing in many of the product images, however, people did not know what it is for or simply did not get one in their shipment. If you look at the photos I've attached one can see that I did indeed get one and figured out its purpose, to remove fur and/or hair from the horizontal brush.
The bottom line is that I would definitely buy this item again.
===========
EDIT, four weeks later: Since replacing the main roller brush with the one included with this product, I have experienced several “Roller brush is stuck” shutdowns and alerts. In each case, there has been nothing tangled in the brush and the brush was free to spin. I have to say it’s quite annoying to come home expecting the floor to be cleaned but only to find this error long before the job was finished. Today it gave this error twice in one cleaning cycle. I never experienced this problem with the OEM roller brush.
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Reviewed in the United States on September 8, 2019
★★★★★ 5
Euphy works good as new!
Style: 19 Pack
As soon as I opened the package everything looked the same- pre-filters, filters, main brush, and side brushes. I changed the big brush first;the only issue was the new brush body didn't have the white arrows to help aligning but that didn't affect how the brush worked. I changed filter and pre-filter, and finally the side brushes.
When I turned on my "Goophy" and tried to let her run the side brushes were a little too (Goophy's side brushes sounded a little louder). I trimmed the side brushes a little and all was well. Goophy didn't want to run herself but it didn't take long for me to figure out it wasn't the new brushes ;just the operator pushing the wrong button. My Goophy Euphy did what she was supposed to do- vacuum the carpet and tile floor. Goophy Euphy still wanted to take the long way to her charging station which was nothing new and easily corrected.
Over all the new brushes and filter restored Goophy Euphy back to when we first used her!
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Reviewed in the United States on July 22, 2023
★★★★★ 4
Don't be cheap like me - buy these and save yourself the hassle!
Style: 19 Pack
I made the mistake of going with another, slightly cheaper set of replacement parts for our Eufy RoboVac 11S. I did not heed the cautionary tales which lead me having to find replacement parts for the replacement parts. This brand fits our vacuum perfectly - the corner brushes don't pop off, the filter is the right size and the roller brush gets sufficiently tangled with cat hair so clearly also a good fit. My only concern (and why I only gave four stars) is - surprise! - the corner brushes. Do they stay on? YES! In fact, they is so secure, I had difficulty swapping one out. Here's the peculiarity of the brand - the "whiskers" on the spinner fell out. I found them all over the floor, on different surface types and under various conditions; they were not getting caught on any one thing. Not all at once, but over two or three uses, one of the corner brushes had become devoid of almost two entire spokes! Ironically, the little guy can't seem to pick up his own mess but I digress. Without the whiskers to grasp, removing the part was difficult. I ended up using a small flathead screwdriver for leverage to pop it off. While the other corner brush appeared to have shed some whiskers as well, it was not in need of replacement. Despite the shedding, this will be my first go-to for replacement parts next go round.
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Reviewed in the United States on September 24, 2022