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Description
P-Selectin/CD62P His Tag Protein, HumanProduct Specification Species Human Synonyms GMP 140, LECAM3, PADGEM Accession P16109 Amino Acid Sequence Trp42 Ala771 with His tag at the C Terminus Expression System HEK293 Molecular Weight 100 120kDa (Reducing) Purity >95% by SDS PAGE, RP HPLC & SEC HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the
Product Specification
| Species | Human |
| Synonyms | GMP-140, LECAM3, PADGEM |
| Accession | P16109 |
| Amino Acid Sequence | Trp42-Ala771 with His tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 100-120kDa (Reducing) |
| Purity | >95% by SDS-PAGE, RP-HPLC & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Weller A, Isenmann S, Vestweber D. Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J Biol Chem. 1992 Jul 25;267(21):15176-83. |
Background
Mouse P-Selectin, alternatively known as GMP-140, LECAM-3, PADGEM, or CD62P, belongs to the Selectin family of cell surface glycoproteins. It is expressed by activated platelets and endothelial cells. The Mouse P-Selectin cDNA encodes a 768 amino acid (aa) type I transmembrane protein, which includes a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain, and a short (35 aa) cytoplasmic domain.
The extracellular domain of Mouse P-Selectin contains an NH2-terminal C-type lectin domain and an EGF-like domain, followed by a series of complement factor A repeat homology domains. It shares approximately 73% sequence homology with the extracellular domains of human P-Selectin.
Mouse P-Selectin plays a critical role in the adhesion of leukocytes and neutrophils to the endothelium. Together with L-Selectin, it initiates the interaction between circulating leukocytes and endothelial cells, leading to the characteristic 'rolling' of leukocytes along the endothelium. This initial interaction is followed by the formation of a stronger bond involving E-Selectin, and subsequently ICAM-1 and VCAM-1. This sequence of molecular interactions facilitates the extravasation of white blood cells through the blood vessel wall and into the extracellular matrix of surrounding tissues.
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