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Description
IdeZ ProteaseProduct Specification Species Streptococcus pyogenes Synonyms IgG endopeptidase Amino Acid Sequence N. A Expression System E. coli Molecular Weight 36kDa Purity >95% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 20 mM Tris HCl, 50 mM NaCl, 1 mM EDTA, pH 7. 5 Reconstitution N. A Stability & Storage 12 months from date of receipt, 20 to 70 C as supplied; 1 week, 2 to 8 C under sterile conditions; Please avoid
Product Specification
| Species | Streptococcus pyogenes |
| Synonyms | IgG endopeptidase |
| Amino Acid Sequence | N.A |
| Expression System | E.coli |
| Molecular Weight | 36kDa |
| Purity | >95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5 |
| Reconstitution | N.A |
| Stability & Storage |
12 months from date of receipt, -20 to -70 °C as supplied; |
| Reference |
1.Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26. 2.Cheng-Wei H, Jie P, Yu-Qing O, et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J]. Chinese Journal of Preventive Veterinary Medicine, 2018. |
Background
IdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.
Protocol
1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.
2. Add IdeZ Protease to the reaction system:
• Add 1 unit of IdeZ Protease per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.
3. Incubate sample at 37°C for 30–60 minutes
Unit Definition
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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