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Description
APC Mouse Anti-Human CD83 Antibody (S-3040)Product Specification Host Mouse Antigen CD83 Synonyms CD83 antigen; hCD83; B cell activation protein; Cell surface protein HB15 Location Membrane Accession Q01151 Clone Number S 3040 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Positive Sample PHA treated Human PBMC Purification Protein G Concentration 0. 2 mg ml Conjugation APC Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12
Product Specification
| Host | Mouse |
| Antigen | CD83 |
| Synonyms | CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15 |
| Location | Membrane |
| Accession | Q01151 |
| Clone Number | S-3040 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | PHA-treated Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD83 is a ~45 kDa, heavily glycosylated type-I transmembrane glycoprotein of the immunoglobulin superfamily that is encoded on human chromosome 6 and expressed chiefly on mature dendritic cells, Langerhans cells and activated lymphocytes, where it serves as a key maturation marker whose extracellular V-set Ig-like domain can dimerize via a membrane-proximal cysteine and whose membrane-bound form drives potent T-cell stimulation while its proteolytically released soluble counterpart (sCD83) exerts potent immunosuppressive effects by inhibiting dendritic-cell maturation and cytokine production, thereby modulating both the initiation and resolution of adaptive immune responses.
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