SKU: 17997778378

Anti-Mouse IgG Acceptor Beads

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Description

Anti-Mouse IgG Acceptor BeadsProduct Specification Stability & Storage Store away from light at 2 8C; product shelf life is 12 months. Background The Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer and luminescence between donor and acceptor beads. Donor beads recognize protein 1 (Tag1 label), while acceptor beads recognize protein 2 (Tag2 label). When protein 1 binds to protein 2, the distance between the beads

Product Specification


Stability & Storage

Store away from light at 2-8°C; product shelf life is 12 months.

Background

The Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer and luminescence between donor and acceptor beads.

Donor beads recognize protein 1 (Tag1 label), while acceptor beads recognize protein 2 (Tag2 label). When protein 1 binds to protein 2, the distance between the beads becomes less than 200 nm. Upon excitation at 680 nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615 nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product offers a simple operation process, requires no washing, and provides fast results with high sensitivity, enabling the detection of weak interactions.

Components

Specification

Fill Volume

250 μg

50 μL

5 mg

1 mL

25 mg

1 mL x 5

Protocol

[Required Reagents]

Name

Catalog No.
Anti-Mouse IgG Acceptor Beads UA086094
Streptavidin Donor Beads UA086104
Universal Buffer 1 UA086113


[Assay Procedure for Reference]

Assay Procedure

Protocol 1 (37℃ Rapid Detection)

Protocol 2 (Room Temperature Detection)

Step 1:

4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Avoid light/Green light

4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Avoid light/Green light

Incubation

 Incubate with shaking at 37℃ for 20 minutes,Avoid light/Green light

Incubate at room temperature for 60 minutes,Avoid light/Green light

Step 2:

Add 6μL Donor Beads,Avoid light/Green light

Add 6μL Donor Beads,Avoid light/Green light

Incubation

Incubate with shaking at 37℃ for 10 minutes,Avoid light/Green light

Incubate at room temperature for 30 minutes,Avoid light/Green light

Reading

Instrument Reading

Instrument Reading


[Performance Validation]

•Sample Preparation:

Pre-dilute biotinylated mouse IgG (Bio-mIgG) to 15μg/mL (100nM) using Universal Buffer 1 as the stock solution, then perform serial dilutions according to the following scheme:

No.

Final Concentration (nM)

Universal Buffer 1

Volume (μL)

High Concentration Add

Volume (μL)

C12

1.0E+01

210

90μL Stock

C11

3.0E+00

210

90μL C12

C10

1.0E+00

180

90μL C11

C9

3.0E-01

210

90μL C10

C8

1.0E-01

180

90μL C9

C7

3.0E-02

210

90μL C8

C6

1.0E-02

180

90μL C7

C5

3.0E-03

210

90μL C6

C4

1.0E-03

180

90μL C5

C3

3.0E-04

210

90μL C4

C2

1.0E-04

180

90μL C3

C1

0

180

/


•Detection Reagent Preparation:

Name

Preparation Concentration

Diluent
Anti-Mouse IgG Acceptor Beads

25 μg/mL

Universal Buffer 1
Streptavidin Donor Beads

25 μg/mL

Universal Buffer 1


•Results for 37℃ Incubation Mode:

Maximum Signal: 5869294

Minimum Signal: 863

EC50= 0.137 nM

•Results for Room Temperature Incubation Mode:

Maximum Signal: 2281873

Minimum Signal: 446

EC50= 0.131 nM

Guidelines


1. This experiment is light-sensitive. Avoid exposure to light during operation. It is recommended to perform preparation, sample loading, and incubation steps under green light (illuminance below 100 LUX).

2. This product is compatible with multifunctional microplate readers equipped with Alpha detection modules.

3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete usage.

4. It is recommended to use the accompanying dilution buffer from our company for reagent preparation and sample dilution. If additional components are required, they can be directly added to this dilution buffer.

5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and time.

6. Avoid generating bubbles during sample loading.

Shipping Notes
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Exchange/Return Notes
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SKU: 17997778378

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Product Reviews
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Dimps
Port Orchard, US
★★★★★ 5
Mind blowing page turner
Oh my stars!!! 🤯 he falls first and he falls hard, enemies to lovers, fated mates. Every characters are lovable. There's action, magic, one horse 🤭 and a plot twist that keep on twisting. Oh and a bad ass FMC! Loved it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2026
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Verified Purchase
Bryan & Lanae Kirby
Battle Creek, US
★★★★★ 3
Interesting. Confusing ending that's too similar to another book
Ok, I had to process almost a whole 24 hours before I could write this review. And long story short, did I enjoy the book, yes. Does it have a lot of interesting and good parts to it? Also yes. But does it also have some major flaws? Absolutely. Now I'm not gonna break down every single little thing in this book. But here are the basics of what I liked, and what I didn't like. The good? I liked the characters. They intrigued me off the bad. The world building is pretty decent. It's a little confusing in the beginning, but information is slowly doled out, and some questions are answered. I found that there was a lot of little twists and turns that kept the story engaging. The magic system is intriguing. But, there are quite a few things off for me. First off, we have another story that has heavy inspirations from other books. There are a lot of aspects in this story that felt directly pulled from throne of glass. And the big twist at the end? Was almost verbatim the same ending as the book how does it feel. As soon as I read it I was like hold up, I literally just read almost this same thing when I read how does it feel when it released like a year or year and a half ago. Now I know no concepts are really new anymore, and inspiration comes for everywhere. But I feel like most stories it's like, oh this book has these vibes, or if you liked this book you'd like this one that's similar. But this reminds me of powerless in where there are like exact plots and plot points taken from other things. Now is that a bad thing? Not necessarily. I still enjoyed powerless, and I still enjoyed this story. But it does throw me. There are also a few red flags that the MMC Acker gave me that were not the good kind of red flags we love. First, when they are riding thru the city and people throw stuff at the FMC and he does nothing? Red flag. When they meet his dad and they demand she vows not only to the king but to the MMC? Red flag. All the secrets he keeps? Red flag. When he SLAPS HER IN THE END? Red flag. I'm not sure how I really feel about him. In the end. This was still an enjoyable read. I did like it and I am curious about the next book. But I am wary about some of the plot points and the MMC.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 26, 2024
S
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SJS
Port Orchard, US
★★★★★ 5
Just read it. You won’t regret it.
Format: Hardcover
This book completely blew me away. I loved every single character—not just the FMC and MMC, but the side characters too, which is rare. In a first book, side characters can feel flat while the focus is on world-building, but that definitely wasn’t the case here. The romantic tension between the FMC and MMC was so good—seriously addictive. The plot itself felt pretty straightforward at first, and the world-building was easy to follow. It even gave me Heartless Hunter / Wolf King vibes… until the last 20 pages. And wow—those final pages. I usually spot plot twists coming, and I thought this one was going to be predictable, but I was completely wrong. That ending genuinely shocked me—I haven’t had that reaction to a book in a long time. I’m already counting down to book two (and that teaser chapter? Absolutely brutal). Just read it. You won’t regret it.
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Reviewed in the United States on May 4, 2026
N
Verified Purchase
Nicole
Alexandria, US
★★★★★ 4
4 Stars for Now!
Format: Kindle
I DEVOURED this book. I was in a huge slump after reading a horrible series right before this but after 3 pages of this book, I was hooked. I read the whole book in 2 days. I think the author did an amazing job at writing an engaging story with an amazing twist at the end. I know many readers feel betrayed by the twist and find the narrator to be unreliable but I feel that the twist was done in a great unsuspecting way. Though I do feel betrayed, I think there is so much room for redemption in the second book! The author also did an amazing job at world/character building. She was able to do so without interrupting the story which is one of my huge pet peeves. A lot of authors build by taking breaks in the plot or dialogue which I find choppy and disengaging but this author was able to build in an engaging way. There was some lack of world/character building but I think that was due to keeping reader in the dark. The world, politics, and characters were all very easy to understand. I found the romance a little lackluster and a bit off-kilter. Some of it was really pushy and rushed which left a bad taste in my mouth. I do hope the author delves deeper into the romance in coming books. The FMC was a breath of fresh air. She was not whiny or stuck-up like most books. She was never afraid to accept help from others but she was still able to shine with her intelligence. Not once did I think she made a poor decision just for the sake of a plot point. She was really easy to like. The MMC could use some work but I think that will happen more in coming books. This is a MUST read, my only advice would be to wait until it is a finished series!
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Reviewed in the United States on July 30, 2025
R
Verified Purchase
Reading_with_Magic
Lowell, US
★★★★★ 5
Need book 2 please :-)
Format: Kindle
From the very first chapter, Metal Slinger hooked me and refused to let go. Set in a brutal, metal-bound world where power is carved from steel and survival depends on strength, strategy, and sheer will, this story follows a young woman forced into a dangerous system where control over metal isn’t just magic — it’s dominance, protection, and rebellion. When she’s pulled into escalating conflict and shifting alliances, she quickly learns that power always comes with a cost. The magic system is sharp and immersive without being overwhelming. The way metal manipulation is woven into combat and politics makes every confrontation feel cinematic and high-stakes. It’s gritty, tense, and beautifully layered. The Brynn, our FMC absolutely shines. She’s fierce but not untouchable, resilient without feeling unrealistic. Watching her navigate betrayal, survival, and the slow awakening of her own strength felt earned every step of the way. There’s grit in her fight scenes and vulnerability in her quieter moments that make her deeply human. And the tension? Immaculate. The chemistry simmers under the surface before sparking into something electric. Every reluctant alliance, every charged interaction, every moment of “are they about to destroy each other or kiss?” had me flying through pages. The pacing is relentless — no filler, no wasted scenes. Twists land hard. Stakes keep climbing. And that ending? Absolutely feral. The kind that leaves you staring at the wall for a minute before immediately checking when book two releases. ✨ Gritty metal-based magic ⚔️ High-stakes survival 🔥 Slow-burn tension 🖤 Fierce, layered heroine 💥 A finale that HITS (but cliffhanger!) This is one of those fantasies that reminds you why you fell in love with the genre in the first place. If this is just Book One, I am more than ready to be emotionally wrecked again in the sequel.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 14, 2026

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