SKU: 18762676375

Mouse IL-6 High Sensitivity ELISA Kit

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Description

Mouse IL-6 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti mouse IL 6 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. IL 6 in the sample binds to the solid phase antibody. After thorough

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- mouse IL-6 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. IL-6 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-6 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

 

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-6 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.43 pg/ml

Detection Range: 1.56-100 pg/ml

Recovery Range: 82-93%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 6 (IL-6)It is a cytokine with both pro-inflammatory and anti-inflammatory properties. Adult mouse IL-6 consists of 187 amino acids and shares 39% and 85% sequence homology with human and rat white IL-6.

During infection and after trauma, especially inflammation caused by burn or other tissue damage, IL-6 will be secreted by T cells and macrophages to promote immune response. Studies have shown that IL-6 is required for mice to resist Streptococcus pneumoniae infection, indicating that IL-6 plays a role in resisting infection.

IL-6 can promote the process of inflammatory and autoimmune diseases in many diseases, such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. Patients with advanced / metastatic cancer have higher levels of IL-6 in their blood.

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SKU: 18762676375

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Marcia Staley Olsen
Natrona Heights, US
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Good quality
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Hunter
Birmingham, US
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Amazing stuff!!
Love love love this stuff! You do have to spread it out well to not go right through it but it doesn’t take more than a thin layer to protect you all day!!
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allentda
Lexington, US
★★★★★ 4
amazing product, a little too pricey
it worked amazing while we were trying to swim in the ocean and snorkel in the ocean. The only downfall I have of this is the price is so much for such a little amount.
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Great clean sunscreen
Great sunscreen, made of minerals instead of chemicals, holds nice in water when surfacing
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Draper, US
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My go to brand
My go to brand. Other brands I have to reapply constantly and still have sunburn. I love this brand and the stick is even better for applying sunscreen. Very reliable without any mess!
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Reviewed in the United States on February 3, 2025

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