SKU: 19457208937

Human IL-4 High Sensitivity ELISA Kit

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Description

Human IL-4 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 4 existing in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.03pg/ml

Detection Range: 0.25-16 pg/ml

Recovery Range: 94-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.

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SKU: 19457208937

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Danny Wilcoxx
Natrona Heights, US
★★★★★ 5
Cute Tray, Bad Charger
Color: Black
Worked for about a month, now it will only charge sometimes. Edit: Seller contacted me and replaced item. It is working perfectly fine now.
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Reviewed in the United States on March 28, 2025
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jessie
Los Angeles, US
★★★★★ 3
The charger is slow
Color: Brown +Wireless
Looks great but the charging takes forever
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Reviewed in the United States on May 18, 2026
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Spunky Punky Sparkle Princess
Cuba, US
★★★★★ 5
These are a good choice
Color: Black-2 Set
These are great. They are made well, look nice and are easy to move when I’m dusting. I use these to keep perfumes, lotions and vitamins organized atop my dresser. The velvety compartments are a well sized and the layout is conducive to storing the kind of objects women or men typically store atop a dresser. I find these bins quite functional. I feel like these were a good value. They weren’t the cheapest and they weren’t the most expensive. They look nice and feel high quality enough.
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Reviewed in the United States on July 4, 2025
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LilyLucy
Phoenix, US
★★★★★ 5
Nice and works great in my nightstand
Color: Beige
Smaller than I expected, but good quality and looks nice in my nightstand drawer. It will keep things from rolling around. Seems sturdy and I like the pinkish undertone to the beige.
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Reviewed in the United States on May 9, 2026
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Mountain Woman
Fort Morgan, US
★★★★★ 5
Great end table organizer, Style, size, cost
Color: Brown-2 Set
Good size, very useful tray. The large compartments are laid out well. Small areas good to hold a watch, change, lip balm, etc. Affordable price and made well, looks more expensive. Keeps "stuff" neat and clean.
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Reviewed in the United States on February 8, 2026

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