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Description
Human Nasopharyngeal Carcinoma Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.4 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 5
Perfect Size and Highly Practical
Color: Black
This sink organizer with sponge and brush holder is the size I was looking for. The non-slip, drain-free base keeps everything dry and in place. Having my sponges and brushes within easy reach makes the sink area much more organized, and it fits perfectly without taking up too much space I like the quality. It has genuinely made my kitchen feel tidier and more efficient.
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Reviewed in the United States on December 4, 2025
★★★★★ 5
Very good quality
Color: Black
It is easy to assemble and works great with my soap, sponge and scrub brush. And it stands stable on the counter beside the faucet. I think the materials used are of good quality and should last a long time.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Love this organizer
Color: Black
Love this thing it’s perfect and makes the sink look pretty and clean very good quality
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Reviewed in the United States on May 15, 2026
★★★★★ 4
Works Good!
Color: Grey
I was concerned it was going to be too small, especially since the tall portion design doesn't allow you to hang on the ourside....But I have a very nice glass container of dish detergent in it, a bottle of hand soap, and the tall container for taller brushes, straw cleaners, etc. It fits everything pretty well. Wouldn't be able to add a sponge to it, but I have a holder with suction cups on the inside of my sink for the sponge and keeps it out of sight, so it works for me. But if you have a stuff at your sink, it probably wouldn't. Though if you like the design (which is very nice), you could always use two, one on each side of the faucet. I also returned one because even though the extra container could hang on the outside, it was short enough and everything kept falling out of it. This taller one works much better...but again, it too is somewhat limited on size and storage. I much prefer everything organized and in its place, and this provides that.
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Reviewed in the United States on April 12, 2026
★★★★★ 5
Great little sink organizer!
Color: Black
This is a lovely addition to my kitchen sink to handle all of my dishwashing tools. And I really like the DE base to catch all the drips and water drainage. The separator in the middle is moveable so you can determine how big or small you want the partitions. It was packaged very nicely and came with stick on feet to protect your countertops or sink edge. Highly recommend if you were looking for something this functional.
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Reviewed in the United States on April 10, 2026