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Description
MBD3 Recombinant Rabbit mAb (S-4367)Product Specification Host Rabbit Antigen MBD3 Synonyms Methyl CpG binding domain protein 3; Methyl CpG binding protein MBD3 Location Nucleus Accession O95983 Clone Number S 4367 Antibody Type Recombinant mAb Isotype IgG Application WB, ICC Reactivity Hu, Ms, Rt, Mk Positive Sample K562, NTERA 2, HeLa, 293T, F9, NIH 3T3, C6, COS 7 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS,
Product Specification
| Host | Rabbit |
| Antigen | MBD3 |
| Synonyms | Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 |
| Location | Nucleus |
| Accession | O95983 |
| Clone Number | S-4367 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | K562, NTERA-2, HeLa, 293T, F9, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
MBD3 is a 32-kDa core subunit of the nuclear Mi-2/NuRD chromatin-remodeling complex that is essential for embryonic development in mice; it binds 5-methyl-cytosine DNA indirectly through a degenerate methyl-CpG-binding domain, couples ATP-dependent nucleosome sliding with histone deacetylase activity, and thereby orchestrates transcriptional repression, pluripotency exit, lineage specification, and heterochromatin formation across vertebrate genomes.
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