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Description
mIHC Dye (TSA480)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 480, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.1 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Holy Grail of Moisturizers
Style: Restorative Protective Cream-3.3 Fl Oz
I bought this after hearing a dermatologist on Instagram rave about this cream and now I see why. As someone who suffers from pretty severe eczema this is my go to holy grail for flair ups. Perfect for sensitive skin and can be used daily. It is very thick so provides a thick layer of moisture on your skin. I will say I have bought 2 sizes in this and the smaller size (1.3fl oz) did separate and had a liquid part that would come out. I have bought numerous tubes of the larger size (3.3fl oz) and never had an issue with separation with the larger size. I’m learning as I get older that more expensive does not mean better quality skincare. This is one that is not only affordable, but will also give you noticeable results. I have everyone in my whole family using this product!
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Reviewed in the United States on May 17, 2026
★★★★★ 5
GREAT STUFF!!!
THIS STUFF IS AMAZING!!! It really does have the consistency of a light mousse, and it goes on so evenly! I have combination skin that changes in dryness and oiliness through the month and by season, but this gives very consistent coverage that doesn't make me break out! It gives my face a matte appearance (but not flat in color) and blends well with my other make up (blush, bronzer, etc.). Great for hiding bumps, acne, and redness too! It also doesn't crease up under my eyes. I am usually the lightest or next to lightest shade in foundations (neutral undertones), and when I first squeezed some out of the tube I thought it would be way too dark/tan for me, but it matches my skin perfectly! The coverage lasts all day for me without reapplication.
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Reviewed in the United States on May 29, 2015
★★★★★ 5
"Blur" is A Good Word For This great Product
This is one of the best BB's I have ever used. I have a strange combination of oily skin that is dry in other areas and also very sensitive. I need something that moisturizes but keeps oil at bay at the same time. SPF is essential and non irritating to my skin and especially my eyes. Kind of hard to find.
PROS:
All of the above was met and it creates a flawless finish. The word "blur" aptly describes the look. I took a picture so you can see the color and how it looks when applied to my hand. Just flawless!
Controls oil
Does not settle into the fine lines around the mouth and eyes.
CONS:
Has a yellow undertone. I have fair skin with a warm undertone so I can wear this, but others may not be able to.
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Reviewed in the United States on February 8, 2016
★★★★★ 4
Awesome product... however...
This stuff is great, it's enough coverage to just throw it on and go. It's so light, and definitely reduces oil, I've only had it for 2 days so I can't say whether or not it makes my skin breakout. But typically la Roche products are awesome on my sensitive combo skin
The only drawback to this product is the fair tone, is suuuppper light. I am blonde haired light skin, green eyed, sorta freckley person. I burn and then tan sorta well but never dark. And the fair/light color ghosts me out quite a bit. But I'm afraid the darker color will be too yellow. I just bought the darker tone. I'll probably mix them.
Contouring the fair tone helps the wash out, but for me, that's not the point of a BB. I just want minimal coverage so I can quickly put it on and not worry about anything.
I think I like the color of the tinted mineral sunscreen better. We'll see about the other tone. Maybe the fair tone will work better for me in the winter.
Over all it's great but I spent $60 on BB cream which is a little outrageous.
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Reviewed in the United States on September 30, 2018
★★★★★ 5
An excellent makeup staple to have in your collection
This is a rich, smooth, creamy SPF foundation that doesn't leave harsh lines on your face from not blending enough. It really blends in with your complexion. This product is also lovely because it has SPF in it, so you are providing sun protection to your face. A little bit goes a long way with this product, so it should last several months of normal use. An excellent makeup staple to have in your collection; one that not only protects your skin but that looks natural and soft.
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Reviewed in the United States on April 4, 2016
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