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Description
Human sPDL1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against solubility programmed cell death protein 1 ligand 1 (sPDL1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of solubility programmed cell death protein 1 ligand 1 (sPDL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Programmed death ligand 1 (PDL1), also known as CD274/, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, CD274 molecule, Programmed cell death ligand 1, and hPD-L1, is a protein encoded by the CD274 gene. Soluble programmed death ligand 1 (sPDL1) has the ability to bind to both PD-L1 and PD-L2 and block the PD-1/PD-L1 interaction. Local delivery of sPDL1 within the tumor microenvironment through local gene therapy has been shown to increase tumor-specific CD8+ T cell immunity and reduce tumor growth. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.7 ★★★★★
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★★★★★ 5
Okay lips… stand UP 💋✨
Color: Light Pink, Size: 0.2 Fl Oz (Pack of 1)
⭐⭐⭐⭐⭐ 5/5 — Glossy, hydrated, and grown-woman cute
This Neutrogena Hydro Boost Tinted Lip Oil is that effortless baddie essential 😌💅🏾 It glides on smooth, feels super lightweight, and gives your lips that juicy, healthy shine without being sticky or messy.
The hyaluronic acid?? Doing the Lord’s work. My lips feel hydrated, soft, and plush — like I drink water and mind my business (even when I don’t 😏). The light pink tint is subtle but pretty, perfect for everyday wear when you want a little something but not a full beat 💕✨
Why it’s a fave:
Hydrates and nourishes 💧
Gives a soft pop of color 🌸
Makes lips look fuller and glossy
Perfect for no-makeup makeup days
This is very much clean girl meets Black Girl Magic energy 🪄💖 Toss it in your bag and reapply whenever you need a quick confidence boost.
If you like your lips shiny, hydrated, and living their best life — add to cart immediately 💕
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Reviewed in the United States on January 15, 2026
★★★★★ 5
A New Love!
Color: Petal Pop, Size: 0.2 Fl Oz (Pack of 1)
I wish I'd known about this before the truckload of nourishing lip glosses I've purchased this year. It's not that they were bad but they didn't deliver the moisturizing I needed. This does it all. It's a slightly tinted doll! The other thing I appreciate is that there's no taste or smell to it. I'm not a big fan of those. It's light and the formula feels good on the lips. Most lip glosses/oils tend to just look pretty without doing anything to the lip in terms of moisturizing or nourishing. This one does! My lips feel soft and subtle and soothed. It lasts a fairly long time too. One other benefit is that it's small enough to tuck into my travel bag so that I can have it on hand for quick touch-ups. The formula is not sticky at all--very smooth. With just a kiss of color, this one is a winner and you can't beat the price.
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Reviewed in the United States on January 4, 2026
★★★★★ 5
Highly recommend for dry lips!!
Color: Radiant Raspberry, Size: 0.2 Fl Oz (Pack of 1), Color: Radiant Raspberry, Size: 0.2 Fl Oz (Pack of 1)
I ordered the radiant raspberry shade and it’s my new favorite everyday lip product. Perfect for everyday use! It’s super hydrating and leaves a beautiful color and shine on your lips. It does transfer onto things just like any glossy product does but when the shine comes off it leaves your lips with a soft cherry tint that looks pretty!
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Reviewed in the United States on May 26, 2026
★★★★★ 4
Great budget-friendly oil!
Color: Radiant Raspberry, Size: 0.2 Fl Oz (Pack of 1)
I have tried a lot of lip oil products and this one ranks up with a lot of designer brands (trust me, I’m Rouge level at Sephora, lol)! I got the raspberry shade and it’s lovely. Lips feel hydrated and it seems to last for a long time for a lip oil-it doesn’t just sink right in and disappear. No scent or taste, and quite a few high-end products can’t state this. This product could easily be a 5 star if not for 2 issues: the doe foot applicator is humongous! Makes a big goopy mess on the lips without much precision, and I have to blot off the edges. Secondly, I don’t like when a product comes without a seal. A lot of products come in a box and don’t have a seal, but considering the ease to which this product is available (from Target to drugstores, etc) and probably bought more often than higher priced oils, it makes me wonder and I hate feeling like I don’t know if someone else has had this item before me. But other than that, this lip oil is tops and I plan on getting another color soon! Get it, you won’t be disappointed.
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Reviewed in the United States on January 5, 2026
★★★★★ 5
Beautiful lip oil
Color: Mocha Mist, Size: 0.2 Fl Oz (Pack of 1)
I just love this lip oil. Not too sticky and gives you great color and coverage. Why spend a ton of money when you can get a great lip oil at a very affordable price. Neutrogena has a winner with this lip oil! Definitely will repurchase in the future.
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Reviewed in the United States on May 15, 2026