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Description
people β2 microglobulin ELISA KitProduct Specification Usage Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at 20 , but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3
Product Specification
| Usage | Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at-20 ℃, but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Operation steps: 1. Dilution of standard product: This kit provides one original standard product. Users can dilute it in a small test tube according to the following chart.
2. Sample addition: Set up blank wells (the blank control wells do not add samples and enzyme-labeled reagents, and the other steps are the same), standard wells, and sample wells to be tested respectively. Accurately add 50uL of the standard on the enzyme-labeled coated plate, first add 40uL of sample diluent to the sample well to be tested, and then add 10uL of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: Incubate at 37 °C for 30 minutes after sealing with a plate sealing film. 4. Liquid preparation: 30 times concentrated washing solution is diluted 30 times with distilled water and used for later use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each hole with washing liquid, let it stand for 30 seconds and then discard it. Repeat this 5 times and pat dry. 6. Enzyme addition: 50uL of enzyme-labeled reagent is added to each well, except for blank wells. 7. Incubation: The procedure is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: First add 50uL of color developer A to each well, then add 50uL of color developer B, gently shake and mix well, and develop color at 37 °C in the dark for 10 minutes. 10. Termination: Add 50uL of stop solution to each well to terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: Zero the blank well, and measure the absorbance (OD value) of each well sequentially at a wavelength of 450nm. The determination should be performed within 15 minutes after the addition of the stop solution. Calculation: Taking the concentration of the standard substance as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find out the corresponding concentration from the standard curve according to the OD value of the sample. Multiply by the dilution factor. Or use the concentration of the standard substance and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply it by the dilution factor, which is the actual concentration of the sample. |
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| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human β2-microglobulin in specimens. The microplate is coated with purified human β2-microglobulin antibody to make solid phase antibody, and β2-microglobulin is sequentially added to the microwells coated with monoclonal antibody, and then combined with HRP-labeled β2-microglobulin antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, substrate TMB is added to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and β2-microglobulin in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human β2-microglobulin in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Synonym | BMG/β2-MG ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 3ug/L-125ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of β2-microglobulin in human serum, plasma and related fluid samples. |
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4.6 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 5
Decent Monitor Stand with Some Assembly Required
Size: Single, Style: Free-Standing
This monitor stand works pretty well and is a good value for the price. It’s sturdy and adjustable, which makes it perfect for a variety of monitors, including my 32-inch curved Samsung. The assembly is straightforward if you have a Phillips screwdriver and something to tighten bolts, like a wrench. It’s solid once it’s put together and holds my monitor securely. The phone holder could use some improvement. It doesn't adjust to face me directly, which is a bit annoying. The stand itself is great for saving desk space and providing better ergonomics, but it can be a bit of a hassle to assemble if you’re not handy with tools. Overall, a good buy if you need a reliable monitor stand.
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Reviewed in the United States on October 25, 2024
★★★★★ 3
Works, Good product, but wouldn't recommend for specifically 3 27inch screens.
Size: Triple, Style: Free-Standing
I purchased this stand to have 3 27inch 2560x1440 monitors, with the side ones angled towards me in a typical 3-monitor setup often seen being used by racing or aviation simulator guys. This stand's arms for the two side monitors are not long enough to have said monitors angled more than 5 to 8 degrees meaning they are forced to be completely linear which is incredibly awkward to try to use because you can barely see or use the screen space on the far edges.
𝐖𝐢𝐭𝐡 𝐭𝐡𝐞𝐬𝐞 𝐬𝐢𝐳𝐞 𝐬𝐜𝐫𝐞𝐞𝐧𝐬, 𝐭𝐡𝐞 𝐨𝐧𝐥𝐲 𝐰𝐚𝐲 𝐭𝐨 𝐡𝐚𝐯𝐞 𝐞𝐯𝐞𝐧 𝐨𝐧𝐞 𝐚𝐭 𝐚𝐧 𝐚𝐝𝐞𝐪𝐮𝐚𝐭𝐞 𝐚𝐧𝐠𝐥𝐞 𝐟𝐨𝐫𝐜𝐞𝐬 𝐭𝐡𝐞 𝐨𝐭𝐡𝐞𝐫 𝐦𝐨𝐧𝐢𝐭𝐨𝐫 𝐭𝐨 𝐛𝐞 𝐢𝐧 𝐩𝐨𝐫𝐭𝐫𝐚𝐢𝐭 𝐦𝐨𝐝𝐞, 𝐤𝐢𝐧𝐝 𝐨𝐟 𝐦𝐞𝐬𝐬𝐢𝐧𝐠 𝐮𝐩 𝐭𝐡𝐞 𝐰𝐡𝐨𝐥𝐞 𝐩𝐨𝐢𝐧𝐭 𝐨𝐟 𝐛𝐮𝐲𝐢𝐧𝐠 𝐭𝐡𝐢𝐬. The product description should not say it can hold 3 27 inch screens because the amount of struggling I had to do to get it to be acceptable was ridiculous.
The stand is... not heavy enough to safely hold monitors of this size either, and additional weight was required ( 2 10lb dumbbells in my case ) configuring the height of your monitors with this stand can be dangerous as well as they could fall down the central post so you have to be extremely careful, but, it is pretty easy other than that.
It is a great option if you don't have a desk with an edge for typical stands ( I don't. ) You can place the stand anywhere, and you don't have to drill through your desk if you really don't want to. The cable clips are a very nice included bonus, and they sent me a replacement without any hassle when I totally effed up my first attempt at setup.
But, I would have shopped harder and got something else if I knew all this prior all the same. Maybe even bought a gigantic curved screen instead.
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Reviewed in the United States on January 8, 2025
★★★★★ 5
Love this cord.
Size: 10 Ft, Color: White
Love how many outlets etc this cord has. Its the perfect next to bed outlet extender I have had.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2026
★★★★★ 5
Tcstei Flat Plug Power Strip – Compact, Safe, and Convenient Extension Cord
Size: 5 Ft, Color: White
Title:
“Tcstei Flat Plug Power Strip – Compact, Safe, and Convenient Extension Cord”
Review:
This 5-foot extension power strip is very practical and space-saving thanks to its flat plug design. It offers multiple outlets, making it easy to connect several devices at once. The build feels solid and it’s great for tight spaces behind furniture or desks. The cord length is convenient for home or office use. Overall, a useful and reliable power strip for everyday needs. ⭐⭐⭐⭐⭐
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Great in small spots
Size: 5 Ft, Color: White
This works great for that small area you need a few extra plugs and some special connections. Easily mounts to wall and very durable and I love tar I can adjust the cord length to it does t look so intrusive
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Reviewed in the United States on May 29, 2026