SKU: 21706163309

Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

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Description

Unione ®️ TR-FRET Human PD1/PD-L1 Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit utilizes homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between PD1 and PD L1. This method enables simple, rapid, and high throughput screening of inhibitors and antibody blockers. As shown in the figure, the interaction between PD1 and PD L1 is detected using an Eu labeled anti Tag1 antibody (TR FRET donor) and an Ac labeled anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.

As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.

Components

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Pembrolizumab

5μM

5μL

10μL

50μL

200μL

-80℃

Tag1-PD-L1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Tag2- PD1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
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SKU: 21706163309

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4.2 ★★★★★
Based on 9 reviews
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Product Reviews
T
Verified Purchase
TRP
Charlottesville, US
★★★★★ 5
My dog loves this
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
Easy to insert bottles into and easy to toss. My dog loves that it both squeaks and crunches. One of his new favorite toys.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 27, 2026
L
Verified Purchase
Lisa Jones
Phoenix, US
★★★★★ 3
Not durable for a large breed
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG, Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
I received this toy about 2hrs ago. My dog tore a hole in it already. Not made for Rottweiler/American Bulldog breed, apparently. 🙂 Love that there isn't stuffing though, at least she's not making a mess, lol!
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Reviewed in the United States on March 22, 2025
T
Verified Purchase
TLC_936
Charlottesville, US
★★★★★ 5
My dog loves it.
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
I thought I was going to have to return this dog toy because my dog was afraid of the toy, especially the squeaker. But after a while, he finally did start playing with it and now he won’t let the other dogs touch his frog. It’s so bad that if they do touch it, he rips it out or their mouth, jumps up into a chair, and lays on top of it so they can’t get it. He still doesn’t like the squeaker if it’s down in front of him, but if I’m in another room, the squeaker makes him come running. He loves the squirrel I got him too. But the material of the frog seems really durable. The part I like the most of that it’s like a puppet and you can stick your hand in it or an empty bottle of water and then Velcro it shut. If your dog doesn’t like the squeaker, there is a pouch in the mouth of the frog that you can access through this velcroed area and cut it out. Definitely a must have if you have a tough chewer.
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Reviewed in the United States on November 3, 2020
T
Verified Purchase
Tina
New York, US
★★★★★ 1
Don't waste your money!
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG, Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
Do NOT buy unless you like throwing away your money! This Ethical Pets 54093 Skinneeez Frog seemed like a great dog toy, but my Malti Poo destroyed it within TEN minutes! He actually ate the material and half of the squeaker before I found the other half. Guess we’ll be seeing that outside in a few days! This toy is listed as “Durable Dog Toy”, but it is NOT. I’ve got dog toys from the dollar store that lasted for months! We will not be buying nor recommending this product again. Don’t waste your money!
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Reviewed in the United States on March 12, 2024
M
Verified Purchase
MGman
Battle Creek, US
★★★★★ 5
Great toy
Color: Grey
My girl loves this toy. She has a lot of toys but this is her favorite. She is a 26 lb mini golden and we have had it over a year. It is just now starting to show wear. Once it is completely worn out I will buy it again!
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Reviewed in the United States on February 20, 2025

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