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Description
Mouse ovary Organoid Culture KitProduct Specification Usage 1. Primary (1) the normal ovarian tissue of mice after sampling must be cleaned at 2 8 , several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the culture dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1 3
Product Specification
| Usage |
1. Primary (1) the normal ovarian tissue of mice after sampling must be cleaned at 2 - 8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the culture dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3 mm3using ophthalmic scissors or scalpel (3) the tissue was digested with the digestive juice C of mouse normal ovary primary tissue, and then was shaken at 37 ℃ for 15-20 min. (the digestion condition was observed at any time during the digestion process) (4) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (6) Matrigel calculation: after step 6, observe the collected tissue volume, add 25-30 times the tissue volume of Matrigel (abs9495) to resuspend the plate (7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add mouse normal ovarian organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul mouse normal ovarian organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal ovarian organoid culture medium a. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.6 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
THIS STUFF WORKS!!!!!!
First of all - RUN, DON’T Walk & get this. IT WORKS!!! I kid you NOT, that I can literally feel sprouts of hair growing out of the roots of my head! After washing my hair And drying it, I part my hair into several sections and gently massage it into my scalp. After at least a day or so, I can feel the tickle in my scalp, it feels like grass slowly growing. I have plenty of hair, but I would love to actually see this product used on someone who doesn’t have hair like me. I am using the product because of a thinning spot that I have from a sew-in that I have had multiple times, and getting it parted in the same area for over a period of time. You can Not beat this product, especially for the price.
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Reviewed in the United States on July 18, 2025
★★★★★ 1
Don't not purchase!!!!
Color: A
This is product has over ingredients, its made in China and the shelf life is 3 years! No naturall product has a shelf life of a 3 years or any storing instructions. Just an FYI when buying any product, the ingredient that's the most in the product is the first on this list of ingredients WATER is the first, Batana Oil is second to the last. the packaging say 100%, 100% what? Its truely not Batana Oil. I am sending this back ASAP and working on my own mix for my hair growth. I am dealing with scared alopecia and have been searching for things to help with my hair growth. I am tried of companies thinking they will take my money. I was not able to see the ingredients until I recieved the package, which is not a terriable way to sell a product.
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Reviewed in the United States on October 5, 2024
★★★★★ 5
We love this stuff
Color: A, Color: A
My baby had an infection in his hair that he got once he started daycare. We tried everything to kill the fungus. It got so bad I had to take him to the doctor and they finally gave him some antibiotics and medicated shampoo. His head was so sore and tinder he didn’t want anyone to touch his head. So imagine trying to wash his hair everyday. The shampoo killed the fugus and took his hair out where it was. I purchased this product. IT REALLY WORKS!!!!!! We’ve been using it a few weeks and we definitely see a difference.
P.S I went and purchased the whole set
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Reviewed in the United States on April 9, 2025
★★★★★ 4
I like it
Color: A
I'm unsure how it affects hair growth since I've only had one jar. It does leave hair moisturizedand soft. It has a really nice scent.
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Reviewed in the United States on May 9, 2025
★★★★★ 5
Love this product!
Color: A
This really keeps the hair moisturized. Don’t use it too much because it causes build up. You can use as a conditioning treatment and wash it out or you can use it as a moisturizer.
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Reviewed in the United States on May 24, 2025
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