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Description
FITC Mouse Anti-Mouse H-2Kd Antibody (S-R647)Product Specification Host Mouse Antigen Mouse H 2Kd Synonyms H 2 class I histocompatibility antigen, K D alpha chain; H 2K(D); H2 K1; H2 K Location Membrane Accession P01902 Clone Number S R647 Antibody Type Mouse mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample BALB c mouse splenocytes Purification Protein A Concentration 0. 05mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin
Product Specification
| Host | Mouse |
| Antigen | Mouse H-2Kd |
| Synonyms | H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K1; H2-K |
| Location | Membrane |
| Accession | P01902 |
| Clone Number | S-R647 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.
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