SKU: 23223567299

Ready to use immunohistochemical secondary antibody kit (anti mouse)

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Description

Ready to use immunohistochemical secondary antibody kit (anti mouse)Product Specification Description packaging: 5ml (can be used for 50 pieces) applicable: primary antibody is from mouse chromogenic substrate: dab (benzidine) reagent composition: component name specification quantity Endogenous peroxidase blocker 5ml 1 HRP enzyme labeled anti mouse secondary antibody polymer 5ml 1 DAB substrate (20) 0. 25ml 1 DAB diluent 5ml 1 Hematoxylin 5ml 1 reagents not provided but required: primary antibody (from mouse), 5%

Product Specification

Description

packaging: 5ml (can be used for 50 pieces)

applicable: primary antibody is from mouse

chromogenic substrate: dab (benzidine)

reagent composition:

component name specification quantity
Endogenous peroxidase blocker 5ml 1
HRP enzyme labeled anti mouse secondary antibody polymer 5ml 1
DAB substrate (20×) 0.25ml 1
DAB diluent 5ml 1
Hematoxylin 5ml 1

reagents not provided but required: primary antibody (from mouse), 5% goat serum (abs933), pbst

this kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time

storage and validity period

2-8° C save. Each component can be stored for at least 12 months



operation steps:

note: all the steps are operated at room temperature. The reagent can be directly titrated in a drop bottle or with a pipette. One drop =30-40ul

1Dewaxed and hydrated tissue sections

2Wash 2-3 times in PBST for 5 minutes

3According to the special requirements of the applied primary antibody, the tissue sections were pretreated (antigen retrieval)

4Wash 2-3 times in PBST for 5 minutes

5Add 100ul endogenous peroxidase blocking agent was incubated for 10 min to block endogenous peroxidase to reduce nonspecific background staining

6Wash 2-3 times in PBST for 5 minutes

7Add 100ul of 5% to 10% normal goat serum and incubate for 20 minutes at room temperature. If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted

8Wash 2-3 times in PBST for 5 minutes

9Add primary antibody and incubate at room temperature or 37 ℃ for 30 min

10Wash 2-3 times in PBST for 5 minutes

11Add 100ul HRP enzyme labeled anti mouse & Rabbit secondary antibody polymer was incubated for 30 min   Note: this liquid is sensitive to light, and keep away from light

12Wash 2-3 times in PBST for 5 minutes

13Use freshly prepared dab chromogenic solution (Dilute with dab Dilution DAB substrate , ready to use) , incubate at room temperature for 5 minutes (the time can be adjusted according to the color development), and rinse the slide with tap water

14Add 100ulhematoxylin , incubate for 3 minutes (the time can be appropriately adjusted according to the color development), and rinse with tap water to return to blue

15Dehydrated, transparent, sealed

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SKU: 23223567299

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KAB
Omaha, US
★★★★★ 5
Great Read!!! Great story!!!
Format: Kindle
The series is long, but Ms. Wolfhart does a fantastic job of weaving this tale while bringing so much to the characters. Surprises and plot twists along the way to keep you intrigued. There is some graphic sex, but is no way the focal point. Grammar was excellent (a rare find with a lot of self publishers) with only a few noted errors. I rarely give 4 stars, let alone 5.
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Reviewed in the United States on April 30, 2021
E
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Elisa
Dallas, US
★★★★★ 3
Sadly, DNF
Format: Kindle
I read this thru KU. I LOVED the synopsis. And then I began reading... and it was a DNF at 68% after picking it up and putting it down several times because I really loved the main female character. *****SPOILERS***** Pros: The world is unique, intriguing and fun. The primary female character is bad-a** but not a b*tech or a mary sue. The primary female has depth. I really want to know what happens to her even tho it's been weeks and I don't remember her name. The villains to the point I read are pretty good -- an ever present threat of mysterious and possibly many culprits. Cons: Way, way too many points of view. I stopped counting at 7. It's the prime reason why I don't care about most of the characters or remember their names even when I like them. There's just too many points of view so almost none of the characters have enough book space for the author to properly develop them. This literally killed the book for me. Actually it killed my desire to read. For weeks. The main male is more villain than hero. He agreed to marry the main female then locks her up & eschews her for her sister, all while bad mouthing her as unfit to rule when he never spent any time with her getting to know her. He is actually unfit to rule as he is blind to the woes of his own kingdom and starts off a peace mission to secure a ceasefire through marriage by murdering an inn full of people in her country for no real reason. Plus, he constantly makes promises he does not keep. And it's gross of him to pine for the sister behind the main female's back. ***** As much as I really wanted to see what happened to the main female character, it wasn't enough for me to keep trying to slog thru this book. There was a lot of potential here that just fell short. Hence, 3 stars.
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Reviewed in the United States on June 19, 2021
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MaryBeth K
Battle Creek, US
★★★★★ 5
Fae Courts with High Intrigue
Format: Kindle
This book is one that just builds and builds and then surprises you to no end. You may think you know the villains and then you are jolted in another direction. Princess Reyna is a real gem, strong of character, a fierce fighter, and loyal to her family and kingdom. Just when you think she and Lorcan, well you know, the plot is flipped. Can't wait to see where this goes in book two.
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Reviewed in the United States on July 7, 2023
K
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Kindle Customer Maureen
Phoenix, US
★★★★★ 4
Slow, sorry but good
Format: Kindle
This was a slow moving book. Lots of character pov chapters, lots of superfluous descriptions and endless courtly appearances stalled this book to start. Once you get into the heart of the story, it takes off. Before you know it the book is done. My favorite character is Reyna. She is so strong. She is true to herself. She gets into a lot of trouble with her headstrong ways but it's entertaining. I have high hopes for Lorcan. He is honorable to a fault. Thane had turned out to be better than I thought but i still don't like him. Eislin is useless. Great plot twists at the end. I'm looking forward too book 2.
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Reviewed in the United States on June 6, 2020
F
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Faifre6
San Leandro, US
★★★★★ 5
Very detailed! Beautiful world building! Strong Heroine!
Format: Kindle
Starts off a little slow and confusing with different POV’s, but starts to all come together towards the middle to make an elaborate plot line and makes it all worth it. Beautiful world building and attention to detail as well as great writing. The cliffhanger was gut wrenching! Can’t wait for the next book!
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Reviewed in the United States on March 4, 2020

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