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Description
Alexa Fluor® 488 Mouse Anti-Rat MHC II (RT1D) Antibody (S-R649)Product Specification Host Mouse Antigen MHC II (RT1D) Location Membrane Accession Q31281 Clone Number S R649 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample SD Rat splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied
Product Specification
| Host | Mouse |
| Antigen | MHC II (RT1D) |
| Location | Membrane |
| Accession | Q31281 |
| Clone Number | S-R649 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD Rat splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
MHC II (RT1D) is a rat major histocompatibility complex class II heterodimeric glycoprotein composed of an α and a β chain that together form an open-ended peptide-binding groove specialized for capturing 12–25 amino-acid fragments derived from exogenous antigens processed through the endocytic pathway; expressed primarily on professional antigen-presenting cells such as B cells, macrophages and dendritic cells, it presents these peptides to CD4⁺ T-helper cells to initiate and shape adaptive immune responses, and in the rat it can be induced by interferon-γ on oligodendroglial progenitor cells, with structural and functional properties that overlap partly with the mouse H2-E and human HLA-DR molecules.
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